胚纹状体移植入Huntington舞蹈病模型鼠脑内的实验研究  

AN EXPERIMENTAL STUDY ON STRIATAL TISSUE TRANSPLANTS INTO BRAIN OF RAT MODEL OF HUNTINGTON DISEASE

作　　者：李军[1] 徐慧君[1] 武义鸣[1] 冯家笙[1] 

机构地区：[1]南通医学院解剖学教研室,南通226001

出　　处：《解剖学报》1993年第1期44-50,T006,共8页Acta Anatomica Sinica

摘　　要：用鹅膏覃氨酸(ibotenic acid)分3点注入SD鼠右侧尾壳核头部,制成Huntington舞蹈病模型。毁损后1个月,用胚龄15～17d同种胚鼠纹状体悬液植入模型鼠侧脑室。实验分正常对照组,模型对照组,单纯胚纹状体移植组(ST组),含基膜蛋白Laminin胚纹状体移植组(LST组)。ST组及LST组于移植后3和6个月的平均主动回避反应阳性率与模型组间均有显著差异;与正常组相比,LST组于移植后3和6个月时均无显著差异,而ST组至6月时方无显著差异。移植后6个月测试动物夜间活动次数,发现模型鼠活动过度,而ST组与LST组与正常鼠近似。动物在存活6个月测试后处死,脑切片观察发现,伤侧尾壳核萎缩,移植组织位于尾壳核背侧并突入侧脑室。LST组移植区大于ST组。两移植区均见ChAT、GABA及Leu-ENK免疫阳性细胞,其形态及大小与正常对照组尾壳核所见相似。LST组AChE阳性细胞突起较ST组多且长。本实验证明,用胚纹状体移植入Huntington舞蹈病模型鼠,移植物能存活、生长并发挥其功能效应;基膜蛋白能促进神经元突起生长。Huntington disease (HD) rat model was produced by injection of ibotenic acid (IA) into the head of right caudate putamen (CP). One month post lesion, fetal (El5-17) striatal cell suspension was implanted into the lateral ventricle ipsilateral to the lesion, and the rats were devided into four groups; group I normal control rats (n=8), group II model control rats (n=10), group III simple transplanted with fetal striatal cell suspension, (n=10, ST group), group IV grafted with striatal cell suspension containing laminin (n=6, LST group). Three and six months after grafting, active avoidence test was carried, the results showed that there were significant differences bewteen grafted groups and model control group at three and six months, and no significant differences between LST group and normal group either in three or six months whereas between ST group and normal group no significant difference only can be found at six months. Overnight locomotor activity was measured in each group at six months post grafting, the results indicated that the locomotor behaviour of model control group was hyperactive whereas the overnight hyperactivity was compensated completely in the grafted groups. After the behavioural test, the rat brain was investigated morphologically. The head of the lesioned CP was atrophied and the graft was located at the dorsal part of the atrophied CP and projected into the lateral ventricle. The volume of the graft area of LST group was larger than that of ST group. ChAT, GABA and Leu-ENK positive neurons were found in the graft area of the two grafted groups and their shape and size were similar to those of nomal CP. The processes of AChE positive neurons in the graft of LST group were more and longer than that in the graft of ST group. The results indicated that the fetal striatal neurons implanted into the lateral ventricle of HD model rats not only can survive and grow well but also ameliorate the behavioural deficits of the IA lesioned rats and the laminin may supports the neuronal suvival and g

关 键 词：脑移植 舞蹈病 胚纹状体 基膜蛋白 

分 类 号：R742.202[医药卫生—神经病学与精神病学]