固定化生长细胞生物催化的若干动态性质  

SOME DYNAMIC CHARACTERISTICS OF IMMOBILIZED GROWING CELLS IN BIOCATALYSIS

作　　者：刘幸[1] 陈惠端[1] 

机构地区：[1]华侨大学化工与生化工程系

出　　处：《Chinese Journal of Catalysis》1994年第4期284-289,共6页催化学报（英文）

摘　　要：通过啤酒发酵实验研究了以海藻酸盐为载体的固定化生长细胞生物催化作用的若干动态特性．在分批出料的填充柱循环反应器中，产物抑制型的啤酒发酵、其Ｓ～ｔ，Ｐ～ｔ曲线与批式发酵罐的Ｘｔ／Ｘｓｔ～Ｋｔ理论曲线相似，Ａ（λ＝５２０ｎｍ）的变化可作为简便的检测方法．发酵周期的缩短呈现τｎ＝ｎ－１（τ１－Ｃ）关系，式中ｎ＝２～４．这对生产上前期的成功应用是有力的支持．但在ｎ≥５时出现载体的崩解和起泡现象，因相催化剂不能继续使用．ome dynamic characteristics of immobilized growing cells in beer fermentation, with alginate as carrier, were studied.A column packed with immobilized Saccharomyces carbergensis was operated in a way of recycling and periodically discharging.The plots of S￣t and p￣t resemble the theoretical plot of Xt/Xst￣Kt in the batch fermenter. So the fact that the 'regular proliferation of cells actually existed was proved. It is shown that the changes of μ,π and ν values were almost synchronous. The change of optical density for the culture could be used as a simple reference of testing method for the similar fermentation process. The reduction of periodic time could be expressed as πn= n-1(π1-C), where n= 2￣4. This is very helpful for the immobilized growing cells to be successfully used in beer production. But the carrier alginate began to be broken down and the bubbling occurred while n was higher than or equal to 5.

关 键 词：固定化生长细胞 啤酒发酵 生物催化作用 海藻酸盐 

分 类 号：TS261.1[轻工技术与工程—发酵工程]