利用离体诱变技术选育小麦大粒种质的研究  

Study on the Selection of Wheat Macro-kernel Germplasm by the Technique of in Vitro Irradiation

作　　者：吕善勇[1] 何世贤[1] 孙桂芝[1] 时香玉[1] 刘金元[1] 

机构地区：[1]山东省农科院原子能农业应用研究所

出　　处：《生物技术》1994年第1期22-25,共4页Biotechnology

摘　　要：以鲁麦１２等为材料进行幼层培养，转分化前辐照１０００ｒａｄ，在Ｒ２代调查千粒重变异情况，结果表明，后代千粒重发生了显著变化，变异范围为３９．５—６８．５ｇ．突变率高达６３．０８％，最高千粒重超过对照４０．９５％。已选出一批大粒材料应用于生产．其中核生二号新品种千粒重６５ｇ，杂交后代表明，大粒变异多为显性突变。The young embryo of 'Lumei No.12' was cultured.Their callus was treated with 60 Co-r rays of 10Gy just before diffarenhation.The variation result of 1000-kernel-wcight in R2 yeneration showed that the 1000-kernel-Wedt changed wiificantly in the progenies with a range of 39.5-68.5g,and the mutation rate ap to 63.08%,the po 1000-kernel-wegh was 40.95% her than that of CK.A few strains with macro-kernel have been selected and applied cd ptoduction.TIle new wanety of'HESHENG No.2' with 1000-kernel-weight Of 65g is one of them.The hybridhation progenies also showed that most macro-kernel variat6ions are dominant mutations.

关 键 词：小麦 幼胚培养 辐照 千粒重 突变 

分 类 号：S512.103.5[农业科学—作物学]