菱叶红景天多糖的提取、纯化鉴定及理化特性研究  

Study on Extraction, Purification Identification and Physical and Chemical Characteristics Assay of Rhodiola Henryi Polysaccharides

作　　者：刘志伟[1,2] 姜华年[3] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所 [2]武汉工业学院食品科学与工程学院,湖北武汉430023 [3]武汉工业学院食品科学与工程学院

出　　处：《食品科学》2005年第3期60-63,共4页Food Science

摘　　要：以有效的热溶水提法,从菱叶红景天植物中得到粗多糖RHP。RHP经乙醇分级得三个级分RHP-I,RHP-II,RHPIII,取RHPIII脱蛋白,脱色,DEAD-纤维素柱层析,Saphadex　G-200柱层析分离纯化,色谱鉴定及初步理化特性分析,得一纯度较高的均一多糖RHPS。PC分析其单糖组成有L-阿拉伯糖、L-鼠李糖、D-葡萄糖和另一个待确定的组分组成。The R. Henryi Polysaccharides (RHP) were isolated from R. henryi by a high efficient water-extrating method. RHP- I,RHP-II and RHP-III were obtained by ethanol precipitating in RHP. After using RHP-III, eliminating albumen, decolorating, dialysis and freeze drying, it was purified by DEAE-cellulose column chromatography. As purified polysaccharides, RHPs were obtained and identified by Saphadex G-200 column chromatography and UV and IR spectrum.The results analysed by paper chromatography showed that its monnosaccharides were arahinose(Ara), rhamnose (Rha) and glucose(Glc), but a monnosaccharide should be further identified.

关 键 词：菱叶红景天多糖 提取 纯化 鉴定 理化特性 

分 类 号：Q539[生物学—生物化学]