部分背根切断对备用背根节神经元Bax、Bcl-2及Caspase-3表达的影响  

Expression of Bax, Bcl-2 and Caspase-3 in Spared Dorsal Root Ganglion after Partial Dorsal Root Rhizotomy

作　　者：徐新运[1] 周雪[1] 王廷华[1] 张连双[1] 赵秀军[1] 刘芬[2] 章为[1] 

机构地区：[1]四川大学华西基础医学与法医学院组织胚胎学与神经生物学教研室,成都610041 [2]昆明医学院神经科学研究所

出　　处：《四川大学学报（医学版）》2005年第2期180-183,共4页Journal of Sichuan University(Medical Sciences)

基　　金：纽约中华医学基金会 (CMB0 0 -72 2 )资助

摘　　要：目的　研究部分去背根对备用背根节 (DRG)神经元 Bax、Bcl- 2、Caspase- 3表达的影响。方法　制作单侧备用背根模型 ,3d、7d和 14 d后取备用背根节分别用兔抗 Bax、Bcl- 2及 Caspase- 3行免疫组化 ABC法染色 ,同时另设一正常对照组。分别计数单位面积内各组大、中小阳性神经元的数量。结果　正常组 Bax、Bcl- 2、Caspase-3阳性反应物主要分布于背根节的中小神经元和少数大神经元。术后 3d,Bax总阳性神经元数较正常组明显增加(P<0 .0 5 ) ,至术后 14 d Bax总阳性神经元数下降并恢复到正常水平。各时间段 Bcl- 2总阳性神经元数无显著性差异。3d组 Caspase- 3总阳性神经元数与正常组比较显著增加 (P<0 .0 5 ) ;7d组、14 d组与 3d组无显著差异。 Bax、Bcl- 2和 Caspase- 3阳性大神经元数在各时间段均无显著性差异 ,阳性中小神经元数的变化规律与总阳性神经元数的变化规律相同。结论　部分去背根主要对备用 DRG中小神经元 Bax、Bcl- 2、Caspase- 3表达有影响 ,三因子表达高峰可能在术后 3～ 7d。Objective Investigating the expression of Bax, Bcl-2 and Caspase-3 in dorsal root ganglion after partial dorsal root rhizotomy in spared root. Methods Adult cats were used. The study comprised a normal control group (n=5) and three expriment groups (n=5 per group). The unilateral root rhizotomy was performed on the 15 cats of the 3 experiment groups. On the 3rd, 7th and 14th days after partial dorsal rhizotomy, the DRG(L 6) of 5 cats per batch were taken and made into frozen sections and were stained under the same condition using specific Bax, Bcl-2 and Caspase-3 antibody by ABC method. The Bax, Bcl-2 and Caspase-3 positive large-, medium- and small- sized neurons in DRG were counted in the 3 experiment groups and in the normal control group. Results In the intact animals of the normal control group, the Bax, Bcl-2 and Caspase-3 immunoreactants were mainly distributed in medium, small neurons and a few large neurons. The number of Bax positive neurons in spared DRG of the 3rd day group increased apparently as compared with that of the normal control group but decreased apparently in the 14th day group and showed no significant differnce when compared versus control(P>0.05). There was no significant difference between the 14th day group and normal control group. Bcl-2 expression in spared DRG displayed no difference among the four groups. The number of Caspase-3 positive neurons in spared DRG of the 3rd day group increased apparently when compared against control(P<0.05), but it was not significantly different from that of the 7th day and 14th day groups(P>0.05). Conclusion Partial dorsal root rhizotomy mainly has an effect on the expressions of Bax, Bcl-2 and Caspase-3 in medium- and small- sized neurons of spared DRG, the peaks of the expressions may appear 3-7 d after the operation.

关 键 词：Caspase-3 BCL-2 正常 阳性神经元 表达 术后 备用背根节 DRG 阳性反应 水平 

分 类 号：R361[医药卫生—病理学]