家蚕微孢子虫16S rRNA基因序列及分子进化  

Sequence and its Molecular Evolution of 16S rRNA Gene of Nosema bombycis

作　　者：潘中华[1] 曹广力[2] 薛仁宇[1] 郑小坚[1] 陈淼[1] 何泽[2] 贡成良[1] 

机构地区：[1]苏州大学农业科学与技术学院,江苏苏州215006 [2]苏州大学生命科学学院,江苏苏州215006

出　　处：《苏州大学学报（工科版）》2005年第1期8-13,共6页Journal of Soochow University Engineering Science Edition (Bimonthly)

基　　金：国家经贸茧丝办风险基金(编号2049)。

摘　　要：通过PCR克隆了家蚕微孢子虫(Nosemabombycis)苏州株的完整16SrRNA基因序列。序列分析表明,16SrRNA基因大小为1235bp,缺乏多个被认为是真核生物序列的区域,序列结构特征更多地类似于原核生物的,在进化上,推测微孢子虫在很早以前与原始真核生物产生分歧;分子进化分析发现,同属的微孢子虫可以处于系统进化树不同的分枝。The complete 16Sr RNA gene of Nosema bombycis Suzhou strain has been cloned by using PCR. Sequence analysis showed that the size of 16S rRNA gene was in 1235 bp, and lacking several regions which regarded as eukaryotic sequence, was more similar with prokaryotes than eukaryotes in sequence structural feature; it could be implied that the microsporidia had split from ancient eukaryotes very early in time on the phylogenetic tree. Molecular evolution analysis indicated that the microsporidia which even belonged to same genus could be classified to different branch.

关 键 词：家蚕微孢子虫 16S RRNA 序列 分子进化 

分 类 号：Q78[生物学—分子生物学]