兆科降纤酶和抗α_vβ_3整合素抗体对牛视网膜血管内皮细胞粘附、移行的影响  

Effects of Zhaoke defibrase and anti-α_vβ_3 mAb on the adhesion and immigration of bovine retinal vascular endothelial cells

作　　者：罗静[1] 姜德咏[1] 唐罗生[1] 朱小华[1] 

机构地区：[1]中南大学湘雅二院眼科,长沙410011

出　　处：《中华眼底病杂志》2005年第2期118-121,共4页Chinese Journal of Ocular Fundus Diseases

摘　　要：目的　探讨蛇毒制剂兆科降纤酶和抗αvβ3整合素抗体 2 3C6对血管内皮细胞粘附移行的影响。　方法　以玻璃粘连蛋白 (Vn)、胶原包被培养皿 ,牛视网膜血管内皮细胞中加入不同浓度的兆科降纤酶和抗 αvβ3整合素抗体 ,孵育 6 0 min后进行细胞粘附分析和移行分析。应用透射电子显微镜 ,对兆科降纤酶和抗 αvβ3整合素抗体干预后的牛视网膜血管内皮细胞进行凋亡检测。　结果　兆科降纤酶和抗 αvβ3整合素抗体均呈剂量依赖性抑制牛视网膜血管内皮细胞对 Vn、胶原的粘附和移行。兆科降纤酶半数抑制浓度 (IC50 )小于 0 .0 5 μmol/ L,而抗 αvβ3整合素抗体的 IC50 高于 2 .5 μmol/ L。 0 .1μmol/ L 的兆科降纤酶即可抑制 81.8%的内皮细胞对 Vn的粘附 ,10 μmol/ L 抗 αvβ3整合素抗体则可抑制 76 .3%。经两者干预后的牛视网膜血管内皮细胞内均可见典型的凋亡小体。　结论　兆科降纤酶和抗 αvβ3整合素抗体能显著抑制牛视网膜血管内皮细胞对细胞外基质的粘附和移行 ,其机理可能在于诱导牛视网膜血管内皮细胞凋亡。Objective To explore the effects of Zhaoke defibrase and anti-α vβ 3 mAb (23C6) on the adhesion and immigration of bovine retinal vascular endothelial cells. Methods The culture dishes coated with vitronectin (Vn) and collagen,assays of adhesion and immigration were performed 60 minutes after different concentration of Zhaoke defibrase and anti-α vβ 3 mAb was added to the bovine retinal vascular endothelial cells. The apoptosis of bovine retinal vascular endothelial cells induced by Zhaoke defibrase and anti-α vβ 3 mAb was detected by electron microscopy. Results Both Zhaoke defibrase and anti-α vβ 3 mAb inhibited the adhesion and immigration of bovine retinal vascular endothelial cells in a dose-dependent manner. The inhibited concentration (IC 50) of Zhaoke defibrase was less than 0.05 μmol/L, while IC 50 of anti-α vβ 3 mAb was more than 2.5 μmol/L. 81.8% endothelial cells adhering to Vn were inhibited by 0 1 μmol/L Zhaoke defibrase, while 76.3% by endothelial cells adhering to Vn were inhibited by 10 μmol/L anti-α vβ 3 mAb. Typical apoptosis cells were found in bovine retinal vascular endothelial cells after affected by Zhaoke defibrase and anti-α vβ 3 mAb. Conclusion Both Zhaoke defibrase and anti-α vβ 3 mAb can significantly inhibit the adhesion and immigration of bovine retinal vascular endothelial cells to extracellular matrix, and the mechanism may lie in inducing the apoptosis of endothelial cells.

关 键 词：ΑVΒ3 整合素 视网膜血管 降纤酶 内皮细胞 抗体 粘附 移行 体能 结论 

分 类 号：R774[医药卫生—眼科]