气相色谱-质谱法同时测定蜂蜜中的双甲脒及其代谢物残留  

Simultaneous Determination of Amitraz and its Metabolite(2,4-xylidine) Residues in Honey by Gas Chromatography -Mass Spectrometry

作　　者：薛晓锋[1] 赵静[1] 邱静[2] 周志强[2] 

机构地区：[1]农业部蜂产品质量监督检验测试中心,北京100093 [2]中国农业大学理学院应用化学系,北京100094

出　　处：《现代科学仪器》2005年第1期65-67,共3页Modern Scientific Instruments

基　　金：国家科技攻关计划-主要肉蛋奶和蜂产品安全限量及控制标准研究资助课题。

摘　　要：建立了一种用气相色谱-质谱法(GC-MS)测定蜂蜜中的双甲脒及其代谢物(2,4-二甲基苯胺)残留的方法。蜂蜜样品用pH为11.0的水溶液溶解,其中的双甲脒和2,4-二甲基苯胺残留用正己烷超声波提取后直接用GC-MS测定。样品添加浓度在10μg/kg～200μg/kg时,双甲脒和2,4-二甲基苯胺的添加回收率在60%～120%之间,检出限均为10μg/kg。A sensitive and simple method was developed for the simultaneous determination of amitraz and its metabolite(2,4-xylidine) residues in honey by gas chromatography -mass spectrometry. The honey samples were dissolved with anaqueous solution at pH 11,and analyte was extracted with n-hexane by sonication. After separation,the organic phase wasanalyzed with GC-MS directly. The method was quantified by ultra an external standard mode. The recovery of 2,4-xylidineand amitraz added to honey at levels of 10μg/kg～200μg/kg were 60%～120%. The limits of detection (LOD) were 10μg/kg for both 2,4-xylidine and amitraz. The method has been successfully applied to administration of amitraz residue inhoney,the results are satisfactory.

关 键 词：双甲脒 2 4-二甲基苯胺 蜂蜜 气相色谱-质谱 

分 类 号：S896[农业科学—特种经济动物饲养]