毛细管区带电泳对单唾液酸神经节苷脂(GM_1)的分离  

Separation of Monosialogangliosides (GM_1) by Capillary Zone Electrophoresis

作　　者：郑永彪[1] 范慧红[2] 徐康森[2] 

机构地区：[1]青海省药品检验所,西宁810000 [2]中国药品生物制品检定所,北京100050

出　　处：《药物分析杂志》2005年第3期300-302,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：北京市自然基金(7032041)

摘　　要：目的:建立毛细管区带电泳法分离单唾液酸神经节苷脂(GM_1)。方法:采用熔融的硅胶毛细管柱(fused-silica capillary)90 cm×75μm(有效长度为83 cm),以含15 mmol·L^(-1)α-环糊精的100 mmol·L^(-1)硼砂缓冲液为运行缓冲液(pH 9.9),分离电压30 kV;检测波长:200 nm。结果:GM_1的2种组分分离良好。结论:本法可用于GM_1的2种组分的分离测定。Objective: To establish a method using capillary zone electrophoresis for separating two forms of monosialogangliosides(GM_1). Method;The separation was performed on a fused-silica capillary 90 cm×75 μm(efficency length 83 cm). 100 mmol·L^(-1) borax buffer containing 15 mmol·L^(-1)α- cyclodextrin(pH 9. 9)was used as running buffer. The separation voltage was 30 kV. The UV detection wavelength was 200 nm. Results; The two forms of GM_1 were successfully separated. Conclusion:This method can be applied to the seperation and the assay of two forms of GM_1.

关 键 词：GM1 单唾液酸神经节苷脂 毛细管区带电泳法 分离测定 缓冲液 检测波长 Α-环糊精 组分分离 熔融 毛细管柱 

分 类 号：R651[医药卫生—外科学] R743[医药卫生—临床医学]