单纯疱疹病毒性角膜炎淋巴细胞活化状态与γ-干扰素、白细胞介素2水平关系的研究  

The relationship between activated lymphocyte and interleukin?、interferon-γ in herpetic simplex keratitis

作　　者：郑金华[1] 王健英[1] 左丽[2] 陈晓钟[3] 

机构地区：[1]贵阳医学院附属医院眼科,550004 [2]贵阳医学院免疫学教研室,550004 [3]解放军44医院,550009

出　　处：《贵州医药》2005年第2期102-106,共5页Guizhou Medical Journal

摘　　要：目的检测复发性单纯疱疹病毒性角膜炎(HSK)患者外周血淋巴细胞亚群及淋巴细胞CD4/HLA-DR+、CD8+/HLA-DR+的表达,测定HSK患者血清中γ-干扰素(IFN-γ)、白细胞介素2(IL-2)水平,研究HSK患者外周血淋巴细胞活化状态与血清中IFN-γ、IL-2水平的关系,探寻HSK复发的分子免疫机理,为临床治疗提供新思路。方法收集复发性单纯疱疹病毒性角膜炎病例22例,设置体检合格的健康人为正常对照组。取患者及正常对照组外周血,应用流式细胞技术对淋巴细胞亚群、淋巴细胞CD4+/HLA-DR+、CD8+/ULA-DR+的表达进行检测;ELISA双抗体夹心法检测患者血清IFN-γ、IL-2水平。结果(1)复发性单疱病毒性角膜炎患者外周血CD3+、CD4+细胞降低,CD8+、CD19+、CD16+56+细胞无明显变化,CD4/CD8降低,CD4+/HLA-DR+降低,CD8+/HLA-DR+升高,血清IFN-γ、IL-2水平降低,与正常对照组比较,差异有显著性意义(P<0.05)。(2)HSK患者CD4+细胞、CD4+/HLA-DR+表达的降低与血清IFN-γ、IL-2水平的改变呈正相关。结论复发性单纯疱疹病毒性角膜炎患者CD4+细胞、CD4+/HLA-DR+表达降低;IFN-γ、IL-2水平降低;CD4+细胞、CD4+/HLA-DR+、IFN-γ、IL-2与单纯疱疹病毒性角膜炎病情的发展、复发关系密切。Objective To investigate the expression of lymphocyte subgroup、 activated lymphocyte and the relationship between activated lymphocyte and interleukin2、interferon-γ in recurrent herpetic simplex keratitis (HSK). Methods 22 patients with recurrent herpetic simplex keratitis were collected, and 18 normal donors as control group. The flow cytometry was used to investigate the expression of lymphocyte subgroup、activated lymphocyte(CD4+/HLA-DR+,CD8+/YHLA-DR+) from peripherial blood and IFN-γ, IL-2 in serum of patients with herpetic keratitis were tested with double antibody ELISA. Results (1)The percentages of CD3+,CD4+ cell from peripherial blood of patients with recurrent HSK were lower than those from control group. The ratios of CD4 / CD8 and the expressions of CD4+/HLA-DR+ were lower, and higher in HSK group. The concentrations of IFN-γ,IL-2 in serum of HSK group were lower than those from control group. The distinction had statistic significance. (2)The reduce of CD4+ cell、CD4+/HLA-DR+ was positive correlation with the change of IFN -γ、IL-2. Conclusion The expressions of CD4+ cell、CD4+ HLA-DR+ and concentrations of IFN-γ, IL-2 in serum reduced was close rel(?) with the develop or recurrence of HSK.

关 键 词：HLA-DR 患者 IFN-γ IL-2 单纯疱疹病毒性角膜炎 CD4^+ 复发性 水平 结论 HSK 

分 类 号：R772[医药卫生—眼科] R392[医药卫生—临床医学]