北京市社区人群胆固醇调节元件结合蛋白-1a基因多态性与血脂水平的关系  

Relationships between Polymorphism of Sterol Regulatory Element Binding Protein-1a(SREBP-1a)Gene and Hyperlipidemia in Beijing Community Resi dents

作　　者：沈小毅[1] 段雪英[1] 刀京晶[1] 朱文丽[1] 李勇[1] 肖颖[1] 

机构地区：[1]北京大学公共卫生学院营养与食品卫生学系,北京100083

出　　处：《中国预防医学杂志》2005年第1期5-9,共5页Chinese Preventive Medicine

基　　金：20 0 0年"达能膳食营养研究与宣教基金"资助

摘　　要：目的　了解SREBP -1a基因-3 6delG多态性在中国汉族人群中的分布情况及其与高脂血症的关系。方法　本研究以北京市西城区厂桥社区人群筛检出的、未经药物治疗的3 2 2例高脂血症患者、5 1例临界高脂血症患者及164名血脂正常者为研究对象,运用聚合酶链反应-限制性片段长度多态性(PCR -RFLP)检测SREBP -1a基因-3 6delG位点多态性。结果　SREBP -1a基因-3 6delG等位基因频率在血脂正常组、临界高脂血症组和高脂血症中分别为9 5 .%、11. 8%和10 . 3 % ,差异无统计学意义(P >0 . 0 5 )。血脂正常组和临界高脂血症患者中血清TC、LDL -C、HDL -C、TG、ApoAI和ApoB水平在不同基因型间差异无统计学意义;在高脂血症患者中,女性-3 6delG携带者血清TC水平低于G + /G +基因型,差异有统计学意义(P <0 . 0 5 ) ,而其余血脂指标在不同基因型间差异无统计学意义(P >0. 0 5 )。结论　SREBP -1a的G + /G +基因型可能与女性高脂血症患者血清TC水平的升高有关。Objective To determine the e ffects of Sterol Regulatory Element Bin ding Protein-1a (SREBP-1a) gene polymorphisms on the serum lipid levels in the m iddle-aged and elder people of China. Methods A total of 537 unrelated individ uals, aged 35 to 81, were selected from Xicheng District of B eijing. For all the subjects the serum lipids profiles were measured and the SR EBP-1a polymorphisms were analyzed (PCR- RFLP). According to serum TG and TC le vels, all subjects were classified into three groups: normolipidemic group (n =164), preclinical hyperlipidemic group (n=51) and hyperlipidemic group ( n=322). Results The frequencies of - 36delG allele of SREBP-1a gene in normolipidemic group, preclinical hyperlipidemic group and hyperlipidemic group were 0.095, 0.118 a nd 0.103 respectively. There were no significant differences in genotype frequenc ies or allele frequencies of the polymorphism among the three groups (P>0.05) . Th e serum TC levels of the individuals carrying G- allele were significantly lower than that of the G+/G+ carriers in the female hyperlipidemic group (P<0.05). Conclusion G+/G+ genotype of SREBP-1a is significantly related to the increased concentrations of serum TC in female h yperlipidemic subjects.

关 键 词：固醇调节元件结合蛋白 社区人群 基因多态性 血脂水平 高脂血症患者 限制性片段长度多态性 不同基因型 中国汉族人群 北京市西城区 聚合酶链反应 等位基因频率 血清TC 位点多态性 LDL-C HDL-C ApoAⅠ 统计学 A基因 分布情况 药物治疗 研究对象 血脂指标 正常组 

分 类 号：R589.2[医药卫生—内分泌]