过表达小鼠ERA蛋白对细胞系的影响  

The Function of Mouse ERA Protein in Mouse Cell Line

作　　者：董轲[1] 陈苏民[1] 纪宗玲[1] 吴元明[1] 陈南春[1] 

机构地区：[1]第四军医大学基础部生物化学与分子生物学教研室,西安710032

出　　处：《科学技术与工程》2005年第6期342-345,共4页Science Technology and Engineering

基　　金：国家自然科学基金(39870380;39670006)全军医药卫生科研基金(98M108)资助

摘　　要：为了研究鼠ERA蛋白对细胞生长状态的影响,构建了可调控诱导表达载体pEGSH-mera,并与受体表达载体pERV3稳定共转染鼠成纤维细胞L-929。应用PonA进行诱导表达后,Western Blot检测其表达水平发现,细胞中鼠ERA蛋白明显增多;用MTT法测定其生长曲线发现,细胞生长加快;应用流式细胞仪检测其细胞周期发现,G2/M期细胞数量明显减少。所以,鼠ERA蛋白在细胞周期中发挥重要作用。To study the function of mouse ERA protein in mouse cell line, inducible mouse ERA expression vector: pEGSH-mERA was reconstructed then, co-transfected L-929 cells with pERV3. After induced by PonA, the level of ERA protein in cells was increasing that detected by Western-Blot; the growth of cells which over-expressed mERA became rapid, and the percentage of G2/M phase cells was decreased markedly. So mouse ERA protein may play important role in eukaryote cell cycle.

关 键 词：小鼠era基因 基因功能 过表达 

分 类 号：Q786[生物学—分子生物学]