Regulation of Bcl-2 Family in TIP30-Induced Apoptosis in Hepatoblastoma Cells  

肝癌细胞凋亡中TIP30对Bcl-2蛋白家族的表达的调节(英文)

作　　者：石梅[1] 王平[1] 张霞[1] 张红卫[1] 吴孟超[1] 张柏和[1] 

机构地区：[1]山东济南山东大学生命科学院,250014

出　　处：《The Chinese-German Journal of Clinical Oncology》2005年第1期11-15,65,共6页中德临床肿瘤学杂志（英文版）

摘　　要：Objective: To investigate the role of TIP30 in the apoptotic signal pathwayin HepG2, and Hep3B and Hu-7 hepatoblastoma cell lines. Methods: In order to confirm whether TIP30conducted Bcl-2 family was involved in apoptosis signal pathway, MTT assay, in situ 3' end labellingof DNA assay and Western blot were carried out to detect the diverse apoptotic function of TIP30and the regulation of Bcl-2 family. Results: TIP30 induced apoptosis as evidenced by morphologicalchanges in hepatoblastoma cells, which was accompanied by up-regulating Bax and Bad proteins andstimulating them from cytoplasm to mitochondria, and down-regulating Bcl-xl, while it had no effecton the level of Bak protein. Conclusion: TIP30 induced apoptosis partly by modulating the proteinlevels of members of Bcl-2 family in hepatoblastoma cells. Elucidating the mechanism by which TIP30induces cell death might establish it as an anticancer factor.

关 键 词：HEPATOBLASTOMA TIP30 p53 BAX BCL-XL BAD BAK 

分 类 号：R735.7[医药卫生—肿瘤] R737[医药卫生—临床医学]