肺癌易感性与p73基因多态性关系的研究  

Association of two genetic polymorphisms in the 5' untranslated region of exon 2 of the p73 gene and risk of lung cancer

作　　者：胡志斌[1] 缪小平[2] 马红霞[1] 谭文[2] 钮菊英[1] 林东昕[2] 沈洪兵[1] 

机构地区：[1]南京医科大学流行病与卫生统计学系,210029 [2]中国医学科学院协和医科大学肿瘤研究所肿瘤医院病因及癌变研究室

出　　处：《中华流行病学杂志》2005年第2期106-109,共4页Chinese Journal of Epidemiology

基　　金：国家"973"重大基础研究基金资助项目(2 0 0 2CB5 12 90 2 ) ;国家自然科学基金资助项目 (3 0 3 712 40 )

摘　　要：目的　探讨中国人群p73基因 5’UTR区域两个单核苷酸多态性 (G4C14 ,A4T14 )与肺癌的关系。方法　采用病例对照研究 ;选择经组织学确诊的肺癌病例 4 2 5例 ,地区、年龄和性别频数匹配的对照 5 88名 ,以聚合酶链反应 单链构象多态性方法进行多态性检测。结果　此两个多态性之间具备完全的连锁不平衡 ,AT(A4T14 )单倍型在病例组显著少于对照组 (0 .2 2 5vs.0 .2 87,P =0 .0 0 18) ,提示变异的AT单倍型对肺癌具有保护作用。与携带p73GC GC单倍型基因型者比较 ,携带GC AT单倍型基因型者肺癌风险降低 30 % (OR =0 .70 ,95 %CI:0 .5 3～ 0 .92 ) ,而携带AT AT单倍型基因型者肺癌风险降低 5 5 % (OR =0 .70 ,95 %CI:0 .2 6～ 0 .80 )。结论　p73基因多态改变可能与中国汉族人群肺癌遗传易感性有关。Objective To study the relationship between two potential functional polymorphisms in exon 2 of the p73 gene and the susceptibility of lung cancer. Methods Genotypes were determined by polymerase chain reaction-single stand conformation polymorphism(PCR-SSCP) method in 425 histologically-confirmed lung cancer cases and 588 cancer-free controls, frequency-matched by age and sex. Results The two polymorphisms were in complete linkage disequilibrium and the frequencies of variant p73 AT haplotype (A4T14) were less commonly seen in the cases ( 0.225 ) than in the controls ( 0.287 ) ( P = 0.0018 ). Compared with the p73 GC/GC homozygotes,both the AT/AT variant homozygotes and GC/AT heterozygotes were associated with a significantly decreased risk [adjusted odds ratio ( OR )= 0.45 ,95% confidence interval ( CI )= 0.26- 0.80 and OR = 0.70 ,95% CI = 0.53- 0.92 ,respectively]. Conclusion These results suggested that this p73 dinucleotide polymorphism might have had a role to play in the susceptibility of lung cancer.

关 键 词：肺癌 P73基因 携带 易感性 单倍型 多态性 GC 基因型 病例 确诊 

分 类 号：R734.2[医药卫生—肿瘤] R512[医药卫生—临床医学]