ENU诱导获得一种短尾小鼠及其突变基因的初步定位  

Generating a Tail-short Mouse Using ENU Mutagenesis and Mapping of the Mutant Gene

作　　者：邵义祥 陈兵 张成香 陈春波 薛整风 李厚达 

机构地区：[1]扬州大学比较医学中心

出　　处：《动物学杂志》2005年第1期8-13,共6页Chinese Journal of Zoology

基　　金："十五"国家科技攻关计划 (No.2 0 0 1BA710B)

摘　　要：用一种化学诱变剂ENU(乙酰基亚硝基脲 )腹腔注射 3 0只 8～ 1 0周龄C5 7BL 6J(简称B6)雄鼠 (G0代 ) ,6周后与同品系正常母鼠配种繁殖后代 (G1代 )小鼠 3 5 1只。对其后代进行筛选获得一种可遗传的显性短尾突变小鼠。为了定位该突变基因 ,运用平均分布于B6和DBA 2 (简称D2 )小鼠常染色体而在这两者间又有差异的 3 9个微卫星对突变小鼠的 (D2×B6)F1代短尾突变小鼠回交D2得到的有短尾表型的[(B6×D2 )F1×D2 ]F2 代小鼠进行基因组扫描。反向运用经典的位置候选基因法 ,将短尾突变基因定位于 1 7号染色体 ,与D1 7Mit3 3的LOD值为 9 0 8。选用该染色体上与短尾表型相关基因Brachyury (T)最近的微卫星D1 7Mit1 43引物扩增 ,在 1 0 9只F2 代短尾小鼠中未发生一例交换 ,表明Brachyury基因是本例短尾突变强有力的候选基因。Thirty male C57BL/6J(B6) mice (G 0) were injected intraperitoneally with the chemical mutagen, N-ethyl-N-nitrosourea(ENU),and then let them mate with normal(non-mutagenized)B6 female mice to reproduce three hundred and fifty-one G 1 mice. An inherited dominant tail-short phenotype mouse was found among the G 1 mice. To determine the position of the mutant gene on the chromosome, 39 microsatellite markers, which were equally distributed throughout the autosome and were polymorphic between B6 and DBA/2(D2), were used to scan genome of the F 2 tail-short mice, which were obtained by back crossbreeding F 1(B6×D2) with D2. By conversely using the classical method of positional candidate gene, results of linkage analysis showed that the tail-short mutant gene was on mouse chromosome 17 and the log odds score (LODS) between the mutant gene and D17mit33 was 9.08. After selectively amplifying the marker D17mit143 that was most proximal to Brachyury (T) gene that related to the tail-short phenotype on the chromosome 17,no exchange was found among one hundred and nine F 2 generation mice. The result shows that Brachyury is a strong candidate gene of the tail-short phenotype.

关 键 词：小鼠 突变基因 表型 候选基因 17号染色体 C57BL/6 正常 后代 回交 配种繁殖 

分 类 号：Q78[生物学—分子生物学] S828[农业科学—畜牧学]