NS-398对结肠癌细胞系HT-29超微结构的影响  

Effect of NS-398 on the Ultrastructures of colon cancer HT-29 cells

作　　者：贾晓青[1] 王菁华[2] 夏光涛[1] 钟宁[1] 李文捷[1] 张尚忠[1] 

机构地区：[1]山东大学齐鲁医院消化科,山东济南250012 [2]北京医院ICU,北京100730

出　　处：《基础医学与临床》2005年第2期156-159,共4页Basic and Clinical Medicine

摘　　要：目的选择性环氧合酶2抑制剂NS 398可抑制结肠癌细胞系HT- 2 9的体外侵袭力,本研究进一步观察其对HT- 2 9细胞超微结构的影响。方法通过扫描电镜观察细胞表面微绒毛,通过激光共聚焦显微镜观察细胞骨架成分F actin。结果未处理的HT- 2 9细胞表面微绒毛和丝状伪足较多,其末端有分叉现象,NS- 398处理后,细胞表面的微绒毛减少、皱缩呈小球样改变,以10 μmol/L处理组明显。荧光标记的F actin较集中地分布于HT- 2 9细胞核周围,呈现“环状”结构,10 μmol/LNS- 398处理后细胞核周围的“环状”结构消失,荧光强度减弱。结论NS- 398可显著改变HT- 2 9细胞的超微结构,这可能是其抑制HT 2 9侵袭力的机制之一。Objective Our previous study indicated that NS-398, a selective cyclooxygenase inhibitor, had an anti-invasive effect on colon cancer HT-29 cells in vitro. In this study we tried to study the effect of NS-398 on ultrastructures of HT-29 cells. Methods Scanning electron microscope and confocal laser scanning microscope(CLSM) were used to observe the microvilli and cytoskeleton component F-actin of HT-29 cells. Results There were a lot of microvilli and filopodia with crotches in distal end on surface of HT-29 cells in scanning electron microscope. After treatment with NS-398 microvilli and filopodia reduced, shortened and shrunk into corpuscule-shaped structure. Meanwhile the volume of cells decreased. Such alterations were most remarkable in HT-29 cells treated with 10 μmol/L NS-398. Fluorescence labelled F-actin was distributed around nuclei to take on annular structure. Annular structure around nuclei disappeared and intensity of F-actin decreased significantly after treatment with 10 μmol/L NS-398. Conclusion NS-398 had obvious effect on ultrastructures of HT-29 cells, which maybe one of its underlying mechanisms involved in the anti-invasive effect on HT-29 cells.

关 键 词：NS-398 HT-29细胞 超微结构 结肠癌细胞 细胞表面 处理 改变 F-ACTIN 微绒毛 细胞核 

分 类 号：R735.35[医药卫生—肿瘤]