Wilms瘤基因WT_1 mRNA的FQ-RT-PCR定量分析研究  

WT_1 mRNA Expression in Wilms Tumor by Fluorescence Quantity Reverse Transcriptase Polymerase Chain Reaction

作　　者：苏诚[1] 叶根榕[2] 李穗生[1] 张志崇[1] 刘唐彬[1] 莫家骢[1] 詹文华[3] 高劲松[4] 

机构地区：[1]中山大学附属第一医院小儿外科,广东广州510080 [2]东莞市人民医院外科,广东东莞523108 [3]中山大学附属第一医院外科,广东广州510080 [4]中山大学达安基因中心,广东广州510080

出　　处：《中山大学学报（医学科学版）》2005年第2期197-199,203,共4页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省科技计划重点攻关基金资助项目(2003C34202)

摘　　要：[目的]探讨WT1 mRNA在Wilms瘤表达的临床意义及其与Wilms瘤生物学性状的关系。【方法】对22例Wilms瘤、瘤旁肾组织及血液标本行WT1 mRNA FQ—RT—PCR定量检测。用SPSS软件进行统计学检验,分析。【结果】肿瘤组织中WT1mRNA表达显著高于肾组织和血液,与年龄、性别、BWT或UWT无关。UH 型高于FH型,而肾组织表达又较血液高,BWT血中WT1 mRNA表达高于正常对照组及UWT组,而UWT与正常表达无差别。【结论】Wilms瘤基因WT1 mRNA表达与Wilms瘤发生发展存在关联,且与肿瘤的病理及预后相关.表达越高病理类型及预后越差,但与WT患儿的年龄、性别、BWT或UWT无关。血WT1 mRNA表达水平可能作为BWT的诊断或高危筛选指标。[Objective] To study the expression level of WT1 mRNA in Wilms tumor by fluorescence quantity reverse transcriptase polymerase chain reaction (FQ-RT-PCR) technique and its relationship with the bimolecular characteristic of WT and clinical features. [Methods] Twenty-two cases of WT (BWT 4, UWT 18, male 12 and female 10) were studied by quantitative determination of WT1 mRNA in tumor tissue, kidney and blood. Steps include: Total RNA study, cDNA synthesis,and FQ-RT-PCR. SPSS is used for statistics. [Result] WT1 mRNA has a higher quantitative expression level in WT tissue over kidney tissue or blood, UH WT over FH WT, blood of BWT over UWT, and Kidney tissue over blood. No difference of WT1 mRNA expression in blood was found between WT patients and normal children. [Conclusion] Expression level of WT1 mRNA in WT tissue play a role in the development of WT and is of relationship with WT pathological type and prognosis. It may become a prognostic factor, but has no relationship with WT patients' age, sex, BWT or UWT. Nevertheless, WT1 mRNA expression level in patients' blood may possibly be an indicator for BWT.

关 键 词：WILMS瘤 WT1 信使RNA 逆转录聚合酶链反应 荧光定量 肾母细胞瘤 

分 类 号：R737.11[医药卫生—肿瘤]