汉族健康人201名的酒精代谢相关酶基因多态型分布  

Polymorphism of alcohol metabolizing-related enzyme genes and its correlation with drinking-behaviors in 201 cases of Chinese Han healthy population

作　　者：曹西蓉[1] 吴德生[1] 

机构地区：[1]四川大学华西公共卫生学院环境卫生学教研室,成都6100433

出　　处：《中华预防医学杂志》2005年第2期84-87,共4页Chinese Journal of Preventive Medicine

基　　金：四川省计划生育委员会基金资助项目 (98 4 1 );美国中华医学基金资助项目(1999)

摘　　要：目的　了解乙醇脱氢酶 2 (ADH2)基因型和乙醛脱氢酶 2 (ALDH2)基因型的分布情况,为筛选高危敏感个体和采取预防措施以减少酒精相关性疾病的发生提供理论基础。方法　问卷调查筛选出居住在四川省的无直接血缘关系的汉族健康个体 201人(男 104人,女 97人 )、采集血样并搜集饮酒行为资料;聚合酶链式反应 限制性片段长度多态性方法测定ADH2、ALDH2基因型。结果　杂合型ADH2与纯合型ALDH2在中国汉族正常人口中占优势(分别为 53. 23%, 68 .16% ); 9种ADH2、ALDH2基因型组合的分布间差异无统计学意义;纯合型ALDH2在具高、中饮酒频率男性中的分布间差异有统计学意义。结论　汉族正常人口中携带酒精相关性疾病易感基因型个体占多数(68. 16% ),应加强监测与预防酒精相关性疾病的工作。Objective To study the distribution of genotypes about alcohol dehydrogenase 2 (ADH2) and aldehyde dehydrogenase 2 (ALDH2) and its relationship with drinking-behaviors in Chinese Han healthy population as to providing a theoretic direction for filtering out high-risk and sensitive individuals and taking preventive measures to decrease the alcohol-related diseases.Methods Using questionnaires to select subjects(201 persons, including men 104,women 97) for collecting blood samples and data about drinking-behaviors.Techniques of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP )were used to detect the genotypes of ADH2 and ALDH2.Results Heterozygous ADH2 and homozygous ALDH2 were the two dominant ones(respectively 53.23%,68.16%).There were no statistical differences among the distributions of nine combinations of ADH2 genotypes and ALDH2 ones.The difference of distribution of homozygous ALDH2 between males having high and middle drinking-frequencies seemed to be statistically meaningful.Conclusion The proportion of individuals carrying about “susceptible genotypes of alcohol-related diseases” in Chinese Han healthy population should be more than one half(68.16%), which calls on reinforcing the surveillance and preventing the alcohol-related diseases.Correlation between genotypes of ADH2 and ALDH2 and alcohol-related diseases should be more important.

关 键 词：相关性疾病 汉族 ADH 酒精代谢 正常人 健康人 相关酶 多态型 基因型 分布 

分 类 号：R394[医药卫生—医学遗传学]