检测A组轮状病毒的微阵列技术初探  

Preliminary Study of Oligonucleotide Microarray for Detecting of Group A Rotavirus

作　　者：黄海燕[1] 韩金祥[1] 王健伟[2] 高雪芹[1] 潘继红[1] 刘毅[1] 朱波[1] 

机构地区：[1]山东省医药生物技术研究中心,济南250062 [2]山东省病毒学研究所

出　　处：《中国生物工程杂志》2005年第3期79-83,共5页China Biotechnology

基　　金：国家"十五"计划资助项目 (2 0 0 1BA80 4A2 2 )

摘　　要：采用荧光染料TAMRA标记上游引物 ,经RT semi nestedPCR扩增A组轮状病毒的保守区域 ,并将扩增产物与自行研制的玻片微阵列进行杂交。经杂交信号扫描分析 ,可简便快速地检出A组轮状病毒 ,并能达到高灵敏性 ,为下步进入临床打下了基础。By using the up-stream prime labeled with TAMRA,the conservative region of group A rotavirus was amplified by RT-semi-nested PCR.Then the PCR product was added to the oligonucleotide microarray to hybridize with it.By scanning the hybridization signals,group A rotavirus can be detected easily,quickly and high sensitively.It would be the cornerstone of the clinical detection in the future.

关 键 词：A组轮状病毒 微阵列技术 检出 临床 杂交信号 荧光染料 玻片 扩增产物 引物 PCR扩增 

分 类 号：Q78[生物学—分子生物学] R573.3[医药卫生—消化系统]