子宫肌瘤雌激素受体亚型mRNA的表达  

Expression of messenger RNA for estrogen Receptor subtypes in uterine leiomyomas

作　　者：张桂香[1] 赵学东[1] 郑纪宁[2] 王文勇[2] 梅立新[2] 许倩[2] 张金环[1] 孙春荣[3] 

机构地区：[1]河北省承德医学院附属医院妇科,承德067000 [2]河北省承德医学院病理学教研室,承德067000 [3]河北省人民医院妇产科,石家庄050051

出　　处：《临床与实验病理学杂志》2005年第1期77-80,共4页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的　探讨雌激素受体亚型的表达与子宫平滑肌瘤发生、发展的关系。方法　将60例子宫肌瘤患者分为AB两组, A组32例<48岁,B组28例>48岁,应用原位杂交技术测定平滑肌瘤组织中雌激素两种受体亚型mRNA的表达,取肿瘤周 围正常的平滑肌作对照。结果　子宫肌瘤组织中ERαmRNA的表达明显高于正常的平滑肌组织,而ERβmRNA的表达降 低;B组ERαmRNA在子宫平滑肌瘤组织中的表达较A组呈下降趋势,而ERβmRNA的表达呈上升趋势。结论　子宫肌瘤的 发生发展与ERαmRNA的高表达有关,亦与ERβmRNA表达减少有关。肌瘤的萎缩可能与ERβmRNA表达的升高趋势及 ERαmRNA表达的下降趋势均相关。Purpose To study relations between the expression of estrogen receptor(ER) subtypes and uterine leiomyomas. Methods Sxity patients with uterine leiomyomas,among them 32 cases were under 48 years old,28 cases were over 48 years old. Leiomyoma tissue samples were collected to be objective group,whereas myometrium tissue samples were collected to be control group. Expression of mRNA for ER subtypes was studied by using the technique of nonradioactive in situ hybridization(ISH). Results Expression of mRNA for ER-α in leiomyomas was higher than that in myometrium. The expression of ER-β in leiomyomas was lower than that in myometrium. In leiomyomas,the expression of mRNA for ER-α among higher age group shown decreasing trend by comparison with that among lower age group,whereas the expression of mRNA for ER-β shown increasing trend. Conclusions In addition to the increase in ERα mRNA expression,the decrease in ERβ mRNA expression may also be related to the development of leiomyoma. Both the decreasing trend of ERα mRNA expression and the increasing trend of ERβ mRNA expression may be related to atrophy of uterine leiomyomas.

关 键 词：高表达 子宫肌瘤 mRNA表达 雌激素受体亚型 瘤组织 ERΒ ERΑ 原位杂交技术 平滑肌 

分 类 号：R737[医药卫生—肿瘤] R322.12[医药卫生—临床医学]