整肠生菌细胞融合子的发酵及产物分析研究  

Fermention of fusant from Bacillus licheniformis and its product analysis

作　　者：胡尚勤[1] 

机构地区：[1]重庆师范大学生命科学学院,重庆400047

出　　处：《工业微生物》2005年第1期33-36,共4页Industrial Microbiology

基　　金：省教委课题;川教科(1995)36号

摘　　要：利用细胞融合技术,将整肠生菌高芽孢形成率的菌株B1和低芽孢形成率和菌数产量都低的菌株B2进行细胞融合,经芽孢数测定和发酵产物测定与遗传分析鉴别,得到了代谢产物量不同于亲本和芽孢形成率高的新菌株B12。该融合子乳酸产量是亲本的1.2～1.6倍,丁二醇产量是亲本的1.5～2.3倍。芽孢形成率是亲本的1.2～2.1倍。The fusant B_~1-2 of high sporulation rate and difference of metabolic products could be acquired by cell-fusion technique from Bacillus licheniformis B_1 with high rate of sporulation and B_2 with low rate of sporulation .The results showed that yield of lactic acid and butanediol of the fusant B_~1-2 were 1.2～1.6 and 1.5～2.3 times than those of the parent cells separatively; the sporulation rate of the fusant B_~1-2 were 1.2～2.1 times than that of the parent cells.

关 键 词：亲本 产量 遗传分析 细胞融合技术 新菌株 发酵产物 融合子 整肠生 鉴别 代谢产物 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]