类高血糖素多肽-Ⅱ对短肠大鼠残留小肠葡萄糖吸收的影响  

Effects of glucagon-like peptide-Ⅱ on residual small bowel absorption of glucose in a rat model of short bowel syndrome

作　　者：陈吉[1] 李杭[1] 吴国豪[1] 江文明[2] 蒋新国[2] 

机构地区：[1]复旦大学中山医院普通外科 [2]复旦大学药学院药剂学教研室,上海200032

出　　处：《肠外与肠内营养》2005年第2期84-87,共4页Parenteral & Enteral Nutrition

摘　　要：目的:研究类高血糖素多肽 Ⅱ(GLP Ⅱ)对短肠大鼠残留小肠葡萄糖吸收的影响。　方法: 75%小肠切除大鼠随机分成空白对照组(SB组)、类高血糖素多肽 Ⅱ组 (SB/GLP Ⅱ组),另设一组正常进食大鼠作为SB组的对照组。术后第 1天起进食标准大鼠饲料,术后第 6天进行体内小肠循环灌流实验,测定大鼠回肠的单位长度及单位重量的葡萄糖吸收数据。　结果:灌洗段回肠长度各组无差异,SB组湿重 /长度比值显著高于正常组,但显著低于SB/GLP Ⅱ组。灌洗段回肠每厘米 45min葡萄糖吸收量正常组和SB组无差异,SB/GLP Ⅱ组显著高于SB组(P<0. 05)。正常组灌洗段回肠每克湿重葡萄糖吸收率显著高于SB组,但SB组和SB/GLP Ⅱ组无差异 (P>0. 05)。　结论:GLP Ⅱ能显著促进短肠大鼠残留小肠单位长度的葡萄糖吸收。Objective: To study the effect of GLP-Ⅱ on residual bowel absorption of glucose in a rat model of short bowel syndrome. Methods: Rats undertaken 75% small bowel resection were randomly assigned to SB group and SB/GLP-Ⅱ group. Another group of weight-matched normal rats(Normal) received normal diet ad libitum was used as control group for SB group. All rats were allowed normal chow on the 1st postoperative day(POD). On the POD6,the intestinal glucose absorption data per unit length as well as per unit weight of ileum were measured by in vivo circulatory perfusion experiment. Results: Length of perfused ileum in 3 groups of rats were not significantly different. Wet weight to length ratio of perfused ileum in SB group was significantly higher than that of normal group,but significantly lower than that of SB/GLP-Ⅱ group. 45min glucose absorption of per cm ileum in SB/GLP-Ⅱ group was significantly higher than that of SB group(P<0.05),but no significant difference between normal group and SB group. Glucose absorption rate per gram wet weight of ileum in normal group was significantly higher than that of SB group, but no significant difference between SB group and SB/GLP-Ⅱ group(P>0.05). Conclusions: In a rat model of SBS, GLP-Ⅱ can enhance the intestinal glucose absorption measured as per unit length of resudial ileum and GLP-Ⅱ manifests a trend to increase the glucose absorption measured as per unit wet weight of resudial ileum.

关 键 词：大鼠 高血糖素 正常 回肠 小肠 葡萄糖 GLP 吸收 SB 多肽 

分 类 号：R574[医药卫生—消化系统]