穿山龙薯蓣皂苷酶解产物的分离纯化  

Separation and purification of dioscin products from enzyme reaction

作　　者：王元好[1] 刘冰[1] 鱼红闪[1] 金凤燮[1] 

机构地区：[1]大连轻工业学院生物与食品工程学院,辽宁大连116034

出　　处：《大连轻工业学院学报》2005年第1期1-3,共3页Journal of Dalian Institute of Light Industry

基　　金：国家自然科学基金资助项目(30371744).

摘　　要：用柱层析法对穿山龙薯蓣皂苷酶解产物进行分离纯化(硅胶柱中以氯仿甲醇洗脱),得到2种单体皂苷和皂苷元。经薄层层析检测,纯化的单体皂苷1为3 (O) (β D 吡喃葡萄糖基) 薯蓣皂苷,得率为样品的33.95%,用高效液相色谱法检测其纯度为93.82%。产物2的结构和特征有待进一步研究。Two dioscin products and aglycone from enzyme reaction are separated and purified by silica gel column gradient elution with the solvent of chloroform and methanol. The product Ⅰ from enzyme reaction is identified to be 3-(O)-(β-D-glucopyranoside)-diosgenin by TLC. The yield of the purified dioscin from enzyme reaction is 33.95%. The purity of 3-(O)-(β-D-glucopyranoside)-diosgenin is about 93.82% detecting by HPLC. The structure and characteristics of the product Ⅱ needs further study.

关 键 词：穿山龙薯蓣皂苷 酶水解 纯化 硅胶柱 薄层层析 高效液相色谱 

分 类 号：Q946.49[生物学—植物学]