茶多酚对高糖时内皮细胞产生活性氧和TGF-β_1的影响  

The effect of tea polyphenols on the production of ROS and TGF-β_1 by endothelial cells in high glucose culture media

作　　者：邓红[1] 李海浪[2] 王凯[2] 曾玉[1] 

机构地区：[1]东南大学基础医学院病理学与病理生理学系,南京现在浙江大学医学院210009 [2]东南大学附属中大医院儿科

出　　处：《江苏医药》2005年第4期252-254,共3页Jiangsu Medical Journal

基　　金：国家自然科学基金(30370658)

摘　　要：目的探讨高糖对内皮细胞产生活性氧和转化生长因子β1(TGF β1)的影响,以及茶多酚的干预作用。方法培养人脐静脉血管内皮细胞(ECV304),分为正常对照组、高糖组、茶多酚组和茶多酚干预组。培养0、12、36 h后,用分光光度比色法测定细胞上清液超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,用RT PCR法测定细胞内TGF β1 mRNA的变化。结果高糖导致内皮细胞SOD活性下降和MDA含量升高,上调TGF β1 mRNA表达,其变化随时间延长更为明显。茶多酚干预可拮抗高糖时内皮细胞的上述改变。结论茶多酚有抑制高糖对内皮细胞的损伤作用。Objective To investigate the effect of high glucose on the production of reactive ~oxygen species (ROS) and transforming growth factor β_1 (TGF-β_1) in human umbilical vein endothelial cells(ECV304),and the intervention of tea polyphenols (TPs). Methods ECV304 was cultured ~in different conditional media and divided into control group, high glucose group, TPs group and TPs treatment group. After 0,12 and 36h, the activity of SOD and the contents of MDA in supernatant were detected by spectrophotometry, meanwhile the mRNA expression of TGF-β_1 mRNA was determined by RT-PCR. Results The activity of SOD was found to be significantly decreased , while the contents of MDA and the expression of TGF-β_1 mRNA increased in high glucose. Such changes ~became more significant as time prolonged.TPs could inhibit all these changes of ECV304 in high ~glucose. Conclusion TPs may diminish the harmful effect of high glucose on the endothelial cells.

关 键 词：TGF-β1 茶多酚 活性氧 高糖 超氧化物歧化酶(SOD) 人脐静脉血管内皮细胞 丙二醛(MDA) RT-PCR法 转化生长因子 mRNA表达 正常对照组 比色法测定 MDA含量 SOD活性 干预作用 分光光度 时间延长 损伤作用 上清液 细胞内 培养 

分 类 号：R575.2[医药卫生—消化系统] R282.71[医药卫生—内科学]