顶空固相微萃取-气相色谱-质谱联用分析干柴胡药材中有机挥发物  

Detecting the Volatile Organic Compounds of Dry Bupleurumwith Headspace Solid Phase Microextraction Coupled toGas Chromatography and Mass Spectrometry

作　　者：曾栋[1] 陈波[1] 姚守拙[1] 

机构地区：[1]湖南师范大学化学生物学及中药分析省部共建教育部重点实验室,长沙410081

出　　处：《分析化学》2005年第4期491-494,共4页Chinese Journal of Analytical Chemistry

基　　金：863"项目"食品安全关键技术"之天然植物功效成分基金资助项目(No2001BA804A21)

摘　　要：采用顶空固相微萃取与气相色谱-质谱联用技术检测分析干柴胡药材中挥发性成分,选用聚丙烯酸酯涂层,就萃取时间、温度、体积、样品量、预热时间及脱附时间等条件进行了优化,结果表明, 30mL萃取瓶里0. 5g样品90℃温度下预热40min后,以85μmPA涂层顶空萃取50min,于250℃脱附5min,测得88个峰,鉴定26种化合物成分。方法所得结果与传统提取方法(蒸馏提取法)比较,相对含量较高的成分一致,方法重现性理想,可应用于柴胡药材挥发性物质的快速分析。Headspace solid-phase microextraction (HS-SPME) with a 85 μ m polyacrylate (PA) -coated fiber was coupled to gas chromatography-mass spectrometry for the direct analysis of volatile organic compounds from dry Bupleurum powder. Experimental conditions including extraction time, temperature, volume, the amount of sample, warm-up time and desorption time, have been optimized. The results showed that 0. 5 g sample was placed in a 30 mL-dosed vial, heated for 40 min at 90° C prior to headspace absorption, and then extracting for 50 min with HS-SPME (85 μ m PA coating), desorpting for 5 min at 250° C, 88 compounds peaks were detected and 26 compounds were identified. The results are in good agreement with those given by a conventional method. The present method can be used for speedy analysis of volatile substance of medicinal materials with good,reproducibility.

关 键 词：顶空固相微萃取 气相色谱-质谱联用分析 有机挥发物 药材 柴胡 气相色谱-质谱联用技术 挥发性成分 聚丙烯酸酯 挥发性物质 检测分析 萃取时间 脱附时间 预热时间 顶空萃取 提取方法 相对含量 快速分析 样品量 mPA 化合物 

分 类 号：TQ461[化学工程—制药化工]