超临界流体萃取柴胡果实精油及其化学成分分析  

Supercritical fluid extraction of essential oil from the fruits of Bupleurum Chinense DC.and their characterization by GC/MS

作　　者：刘玉法[1] 阎玉凝[1] 刘云华[2] 武莹[1] 魏太明[1] 杨瑶君[1] 贾红婷[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]山东师范大学化学化工学院,山东济南250014

出　　处：《中医药学刊》2005年第4期663-665,共3页Study Journal of Traditional Chinese Medicine

摘　　要：目的:提取柴胡果实精油并分析其化学组成。方法:采用超临界流体萃取技术(SupercriticalFluidExtraction ,简称SFE)提取柴胡果实的精油,气相色谱-质谱联用仪(GC -MS)分析和鉴定其化学成分。结果:精油的提取率为6. 60 % (V/W) ,确定了73个化合物。结论:首次采用超临界萃取法从柴胡果实中提取精油,精油的提取率是柴胡根的3倍多,亚油酸、鲸蜡醇乙酸酯、亚油酸乙酯、(-)石竹烯氧化物的相对含量分别为3 4.85 %、16 .63 %、2 . 64 %、1. 81% ,植醇、鲸蜡醇乙酸酯、亚油酸乙酯、(-) -石竹烯氧化物为首次从该属植物中发现。Aim To analyze the chemical constituents of essential oil from the fruits of Bupleurum Chinense DC..Methods Essential oil from the fruits of Bupleurum Chinense DC.was extracted by using supercritical fluid extraction (SFE),and the chemical constituents to be identified by GC/MS spectrum.Results the yield of essential oil from the fruits of Bupleurum Chinense DC.by SFE was 6.60%(V/W) and 73 components were identified in this oil.Conclusions The essential oil from the fruits of Bupleurum Chinense DC.was produced for the first time,and its yield was 3 times more than its root,the relative contents of linoleic acid? cetyl acetate?ethyl linoleate?(-)-caryophyllene oxide are 34.85%?16.63%?2.64%?1.81% apart in this essential oil.Phytol ?cetyl acetate?ethyl linoleate ?(-)-caryophyllene oxide were confirmed for the first time from the genus of Bupleurum.

关 键 词：柴胡 果实 超临界革取 精油 气相色谱-质谱联用 

分 类 号：R-05[医药卫生]