酸性蛋白酶高产菌株6042（Aspergillus niger 6042）的选育和形态鉴定  

The selection and morphological identification of the 6042 mutant strain of acid proteas

作　　者：钱玉英[1] 冯观泉[1] 费笛波[1] 叶玲玲[1] 章锦秋[1] 何福恒[1] 

机构地区：[1]浙江省农业科学院微生物研究所

出　　处：《浙江农业学报》1994年第4期253-256,共4页Acta Agriculturae Zhejiangensis

摘　　要：由黑曲霉ＣＰｕ菌株用６０Ｃｏγ射线诱变处理，经初筛、复筛，获得５株酸性蛋白酶变异菌株。其中６０４２菌株的形态变异株酸性蛋白活力比出发菌株提高近４倍，经多次传代酶活力稳定。用福林法测定，酸性蛋白酶活力高达２．３万ｕ／ｇ曲。经形态鉴定菌落大小和孢子颜色与原始菌株有明显差异。Five strains of acid protease are obtained by the method of 60Coγ-rays inducing treatment on the Aspergillus niger cellulase strain CPu.It is morphologically identified that the object strain is created,and the enzyme activity is stable through generations Analysed by the Folin Method,the greatest activity of the acid protease reaches 23,000u g.Significant differences exist bctween the mutant strain and the original one in terms of colony size as well as colour of spores.

关 键 词：蛋白酶 菌株诱变 酸性蛋白酶 变异菌株 

分 类 号：TQ925.2[轻工技术与工程—发酵工程]