两种类型人碱性成纤维生长因子的体外稳定性研究  

The Stability of Human Basic Fibroblast Growth Factor in Solution

作　　者：李国辉[1] 张玲[1] 谢秋玲[1] 李志英[1] 洪岸[1] 

机构地区：[1]暨南大学生物工程研究所教育部基因组药物研究中心广东省生物工程药物重点实验室,广州516032

出　　处：《中国生物工程杂志》2005年第4期38-42,共5页China Biotechnology

基　　金：国家"十五"重大专项资助项目(2002AA2Z3344)广东省"十五"重大科技专项资助项目(2001A1090208)

摘　　要：目的:通过野生型bFGF和改构型bFGF蛋白溶液中的聚集过程的比较,初步探讨bFGF在水 溶液中发生聚集的机理。方法:在选择合适溶液体系后,采用蛋白溶解度和促有丝分裂活性能力为 指标,表征野生型bFGF和突变型bFGF聚合程度,分析共价聚合和非共价聚合所起的作用。结果: 在相同的溶液体系中,野生型bFGF的聚集程度高于突变型bFGF。bFGF聚合程度与浓度有依赖性。 沉淀结果分析非共价聚合占主要作用。结论:野生型bFGF在溶液中共价聚和和非共价聚合同时发 生,两种bFGF聚合过程中非共价聚合占较大比例。突变半胱氨酸可以减少聚合发生。Objective investigate the mechanism of aggregation of bFCF in solution by comparing the aggregation of bFGF and its mutant. Methods: in favorable condition, aggregation of bFGF were showed by the solubility of hbFGF protein in solution and its mitogen in vitro, including non-covalent and covalent aggregation. Results: in the same preserved system, the degree of aggregating of wild-type hbFGF is obviously higher than its mutant. Aggregation in solution depend on its concentration, the higher bFGF protein in solution, the more aggregates. A large proportion of aggregates were induced by non-covalent interaction. Conclusions: non - covalent and covalent aggregation was formed simultaneously, non-covalent interaction play a major role. The mutant which Cys sites is replaced with serines may lessen the aggregation.

关 键 词：碱性成纤维生长因子 稳定性研究 BFGF 体外 类型 溶液体系 聚合程度 蛋白溶解度 野生型 聚集过程 蛋白溶液 有丝分裂 聚合过程 半胱氨酸 突变型 共价 水溶液 性能力 依赖性 大比例 

分 类 号：Q516[生物学—生物化学] S852.2[农业科学—基础兽医学]