环氧合酶-2抑制剂celecoxib抑制胃癌生长的实验研究  

Inhibition effects of celecoxib on the growth of gastric carcinoma in vitro and in vivo

作　　者：兰春慧[1] 房殿春[1] 向德兵[2] 贺志高[3] 陈东风[4] 樊丽琳[4] 

机构地区：[1]中国人民解放军第三军医大学附属西南医院全军消化专科中心,重庆400038 [2]中国人民解放军第三军医大学大坪医院野战外科研究所肿瘤中心病理科 [3]中国人民解放军第三军医大学大坪医院野战外科研究所第二研究室 [4]中国人民解放军第三军医大学大坪医院野战外科研究所消化科

出　　处：《胃肠病学和肝病学杂志》2005年第2期134-136,共3页Chinese Journal of Gastroenterology and Hepatology

基　　金：国家自然科学基金资助项目(39870345);全军"十五"重点基金资助(01Z075)

摘　　要：目的研究celecoxib在体内外对胃癌增殖及凋亡的影响。方法不同浓度celecoxib处理体外培养的SGC790 1细胞后,采用噻唑蓝(MTT)法检测处理后2 4、48、72、96小时SGC790 1细胞的增殖活性,流式细胞仪测定细胞周期和细胞凋亡。利用SGC790 1胃癌细胞株建立裸鼠胃癌种植瘤模型,实验组予以celecoxib 10mg/Kg/day灌胃,共给药6周。结果2 5 ,5 0 ,10 0 ,2 0 0 μmol/Lcelecoxib处理SGC790 1细胞2 4、48、72、96小时后,细胞增殖明显受到抑制,呈时间和剂量依赖性特点。5 0 μmol/Lcelecoxib处理SGC790 1细胞2 4h后,流式细胞仪细胞周期分析表明G0 /G1期细胞百分率上升,S期和G2 /M期细胞百分率下降。流式细胞仪检测实验组的凋亡率明显高于对照组( 2 0 4±2 8%vs 7 6±0 6% ,P <0 0 5 )。celecoxib治疗组裸鼠胃癌种植瘤重量为( 0 43±0 2 1)g ,明显低于对照组( 1 3 3±0 45 )g(P <0 0 5 )。结论体内及体外实验表明,celecoxib能有效抑制胃癌生长,其机制可能与其阻滞细胞周期及诱导凋亡有关。Objective To investigate the effect of celecoxib on proliferation and apoptosis of human gastric carcinoma (GC) cell line as well as the growth of GC xenografts in nude mice.Methods SGC7901 cells were treated with celecoxib at serial concentrations of 25,50,100, and 200 μmol/L.The proliferative status of SGC7901 was measured by using methabenzthiazuron(MTT) assay. Cell cycle was determined using Flow Cytometry(FCM) analysis. Nude mice bearing xenografis of the cell line were treated with celecoxib daily until six weeks after tumor implantation. Results After SGC7901 cells were exposed to celecoxib (25,50,100, and 200μmol/L) for 24, 48, 72, 96 h, celecoxib displayed a strong growth effect in a dose-and time-dependent manner against SGC7901 cells. After exposure of the cells to 50 μmol/L celecoxib for 12h, FCM analysis showed the G0/G1 phase ratio increased, and S and G2/M phase ratio decreased. The apoptosis rate was higher in treat ment group than that in control group( 20.44±2.8 % vs 7.6 4±0.6%,P<0.05). At necropsy, in mice given celecoxib, the mean tumor weight were significantly lower than that in control group [(0.43±0.21) g vs (1.334±0.45) g, P<0.05].Conclusions Celecoxib can effectively inhibit the growth of GC both in vivo and in vitro. The mechanisms of antineoplastic effect action may involved in inhibiting various phases of cell cycle kinetics and inducing apoptosis of tumor cells.

关 键 词：胃癌 CELECOXIB 增殖 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]