维生素C和E对Klebsiella pneumoniae合成1,3-丙二醇的调控  

Regulation of Vitamin C and Vitamin E on the Biosynthesis of 1,3-Propanediol by Klebsiella pneumoniae

作　　者：张延平[1] 杜晨宇[1] 饶治[1] 曹竹安[1] 

机构地区：[1]清华大学化学工程系生物化工研究所,北京100084

出　　处：《过程工程学报》2005年第2期197-200,共4页The Chinese Journal of Process Engineering

基　　金：国家'十五'科技攻关项目(编号:2001BA708B01-04);国家重点基础研究发展计划(973计划)资助项目(编号:2003CB716000)

摘　　要：通过外源添加还原剂的方式调控细胞内NADH/NAD再生系统的状态,研究了40～150 mg/LVC及20～100mg/LVE对Klebsiella penumoniae合成1,3-丙二醇的影响,发现外源添加150 mg/L VC或30 mg/L VE均可使1,3-PD合成浓度提高20%～30%;但同时也提高了某些副产物的合成浓度,对代谢流分布的调控作用不明显;1,3-丙二醇得率稍有提高但不显著.提高1,3-pD得率宜从代谢节点(丙酮酸)通量调节方面考虑.Glycerol could be transformed to 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae. Since NADH was required in the process, reductants were considered to affect the formation of 1,3-propanediol. In this work, 40~150 mg/L VC or 20~100 mg/L VE was added into the culture medium to regulate the intracellular redox state (NADH/NAD) of Klebsiella pneumoniae, and the effects of these two reductants on the metabolism of Klebsiella pneumoniae were investigated. It was shown that the concentration of 1,3-propanediol increased by 20%~30% by addition of 150 mg/L VC or 30 mg/L VE into the culture medium. With the increasing 1,3-PD, some byproducts such as ethanol and 2,3-butanediol increased correspondingly, which resulted in an unconspicuous improvement of the yield of 1,3-PD. Rather than addition of these reductants, regulation of metabolic flux was considered to be a feasible attempt to improve the yield of 1,3-PD.

关 键 词：还原剂 维生素C 维生素E KLEBSIELLA PNEUMONIAE 1 3-丙二醇 还原型二磷酸嘧啶核苷酸 

分 类 号：TQ923[轻工技术与工程—发酵工程]