淡水鱼类微囊藻毒素去毒酶基因的克隆  

Cloning of the microcystin-detoxifizyme gene in the main Chinese freshwater fishes

作　　者：廖婉琴[1] 梁旭方[1] 王琳[1] 方玲[1] 雷腊梅[1] 韩博平[1] 

机构地区：[1]暨南大学生命科学技术学院,广州510632

出　　处：《生态科学》2005年第1期6-8,共3页Ecological Science

基　　金：广东省水文局蓝藻重点项目;广东省自然科学基金项目(031886);教育部留学回国人员科研启动基金项目

摘　　要：微囊藻毒素去毒酶在鱼类微囊藻毒素去毒过程中起着关键作用,研究成功克隆鲢鱼、鳙鱼、草鱼、鲫鱼、鳜鱼、罗非鱼等淡水鱼类微囊藻毒素去毒酶基因cDNA核心片段而首次获得这些淡水鱼类微囊藻毒素去毒酶氨基酸序列。鲢鱼、鳙鱼、草鱼、鲫鱼、鳜鱼、罗非鱼微囊藻毒素去毒酶基因与人、小鼠、大鼠、牛、猪、羊的谷胱甘肽S-转移酶基因氨基酸同源性为60%左右,表明淡水鱼类微囊藻毒素去毒酶基因在进化上变异性较大,与其承担微囊藻毒素去毒代谢之特殊功能相适应。Microsystin-detoxifizyme gene plays an important role in microcystin detoxication in freshwater fishes. cDNA fragments that encoding partial sequences of microsystin-detoxifizyme gene were successfully amplified by RT-PCR using degenerate primers from the liver of the main Chinese freshwater fishes, including silver carp, bighead carp, grass carp, goldfish, Chinese perch and Tilapia. Homologous analysis show that the deduced amino acid sequences of the microsystin-detoxifizyme gene in silver carp, bighead carp, grass carp, goldfish, Chinese perch and Tilapia were ~50%-60% homologus to the glutathione S-transferase gene of mammals, such as human, mouse, rat, cow, pig and sheep. The homologous analysis results indicate a big difference between the microcystin-detoxifizyme gene of freshwater fishes and the glutathione S-transferase gene of mammals, which may explain the special function of this gene in freshwater fishes for microcystin detoxication.

关 键 词：微囊藻毒素去毒酶 微囊藻毒素 去毒 淡水鱼类 

分 类 号：Q785[生物学—分子生物学]