以基因芯片技术研究胸腺类癌引起异位ACTH综合征的基因表达  

Gene expression profile in thymic carcinoid tumors with ectopic ACTH syndrome by gene chip technique

作　　者：毕宇芳[1] 叶蕾[1] 宁光[1] 江凌[1] 范惠咏[2] 赵春军[2] 张济[2] 王侃侃[2] 戴蒙[1] 孙首悦[1] 赵咏桔[1] 陈中元[3] 金晓龙[4] 李小英[1] 王卫庆[1] 

机构地区：[1]上海第二医科大学附属瑞金医院内分泌代谢病科 [2]上海血液学研究所 [3]上海第二医科大学附属瑞金医院心胸外科 [4]上海第二医科大学附属瑞金医院病理科

出　　处：《中华内分泌代谢杂志》2005年第2期135-137,共3页Chinese Journal of Endocrinology and Metabolism

基　　金：上海市教委重点学科(2003 44)基金资助

摘　　要：目的　研究引起异位ACTH综合征的胸腺类癌组织与正常胸腺组织的基因表达差异,探讨胸腺类癌引起异位ACTH综合征的发病机制。方法　利用基因芯片技术,通过不同荧光(Cy3和Cy5)标记的对照和胸腺类癌组织RNA样本与芯片杂交,观察两种组织间的基因差异表达。结果　研究发现,基因芯片的4 224个基因中403个基因在胸腺类癌组织中表达下调, 394个基因表达上调2倍以上(其中23个与细胞分裂有关), 51个基因上调5倍以上(其中与细胞分裂有关的基因1个,即PAK3)。结论　引起异位ACTH综合征的胸腺类癌组织与正常胸腺组织存在多个差异表达的基因,PAK3可能参与了该肿瘤的发生。Objective To analyze the differential gene expression between thymic carcinoids and normal thymic tissues and to study thepathogenesisofectopicACTH syndrome caused by thymic carcinoids. Methods Using gene chip technique, the gene differential expression of 2 tissues were observed following RNA labeled with different fluorescences (Cy3 and Cy5) hybridized to gene chip. Results Among 4224 genes on gene chip, 394 were up regulated more than 2 folds in thymic carcinoid tissues, 23 of which were associated with cell mitosis; 51 genes were upregulated more than 5 folds, 1 of which (PAK3) was associated with cell mitosis. Conclusion A group of differentially expressed genes were observed between the thymic carcinoids and normal thymic tissues.These overexpressed and cell mitosis-associated genes probably play a role in the pathogenesis of thymic carcinoid tumors.

关 键 词：基因芯片技术 胸腺类癌 异位ACTH综合征 基因表达 细胞分裂 

分 类 号：R736.3[医药卫生—肿瘤]