高效液相色谱法同时测定拳参药材中没食子酸和绿原酸的含量  

HPLC Determination of Gallic Acid and Chlorogenic Acid in Polygonum bistorta L.

作　　者：刘瑞[1] 何方奕[1] 李磊[1] 李发美[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《药物分析杂志》2005年第4期390-393,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立同时测定拳参药材中没食子酸和绿原酸含量的反相高效液相色谱法。方法:采用 Diamonsil C_(18)色谱柱(5μm,250 mm×4.6 mm),以甲醇-0.4%醋酸水溶液(25:75)为流动相,流速为0.8 mL·min^(-1),柱温为室温,检测波长为290nm。结果:样品中没食子酸和绿原酸回收率分别为95.7%-104%和96.0%-100%;没食子酸和绿原酸的线性范围分别为5.3-53μg·mL^(-1)(r=0.9996)和25-150μg·mL^(-1)(r=0.9998)。结论:该方法可用于拳参药材的质量控制,方法简便、准确,重现性好。Objective:To develop an HPLC method for the determination of gallic acid and chlorogenic acid in Po- lygonum bistorta L.Method:A Diamonsil C_(18) column(5 μm,250 mm 4.6 mm) was used with a mobile phase of methanol-0.4% acetic acid(25:75)and flow rate of 0.8 mL·min^(-1).The detection was set at 290 nm and the column temperature at room temperature.Results:The recoveries of gallic acid and chlorogenic acid were 95.7%- 104% and 96.0%-100%,respectively.The linear ranges were 5.3-53 μg·mL^(-1) with a regression coefficient of 0.9996 for gallic acid and 25-150 μg·mL^(-1) with a regression coefficient of 0.9998 for chlorogenic acid.Conclu- sion:The method described in this paper is simple and accurate with good reproducibility and can be adopted for the quality control of Polygonum bistorta L.

关 键 词：没食子酸 同时测定 药材 拳参 反相高效液相色谱法 C18色谱柱 绿原酸含量 检测波长 线性范围 质量控制 流动相 水溶液 回收率 重现性 

分 类 号：R284.1[医药卫生—中药学] R282.71[医药卫生—中医学]