利用原生质体诱变育种选育富硒能力强的酵母菌株  

Breeding Selenium-Enriched Yeast by Protoplast Mutagenesis.

作　　者：高玉荣[1] 李大鹏[1] 

机构地区：[1]黑龙江八一农垦大学食品学院,大庆163319

出　　处：《微生物学通报》2005年第2期10-14,共5页Microbiology China

基　　金：黑龙江省教育厅科学研究项目(No.9553017)

摘　　要：利用原生质体诱变育种技术选育富硒能力强的酵母菌株,从13株啤酒酵母中筛选出一株富硒量高的诱变出发菌株,采用溶壁酶进行破壁,确定了原生质体制备的最适条件为酶浓度1g/100mL,酶解处理时间为120min,原生质体形成率为95.2%,再生率为21.8%,诱变后筛选出富硒量为821mg/kg,酵母干菌体收获量为0.88g/100mL的酵母菌A1。This paper studied on breeding selenium-enriched yeast by protoplast mutagenesis. A strain which the content of selenium is the highest is selected from thirteen strains yeast. The optimum conditions to form protoplast are lysed by 1 g/100 mL lywallzyme for 120 min, the formation and regeneration being 95.2% and 21.8% respectively. By mutating breed a strain of A1 which the content of selenium is 821 mg/kg and the amount of dry cell of 0.84 g/100 mL is obtained.

关 键 词：原生质体 诱变 育种 富硒 

分 类 号：R151[医药卫生—营养与食品卫生学]