B.licheniformis NG521-1发酵培养基优化的研究  

Optimization of the fermentation medium of B. licheniformis NG521-1

作　　者：吴晟旻[1] 范伟平[1] 

机构地区：[1]南京工业大学制药与生命科学学院,江苏南京210009

出　　处：《南京工业大学学报（自然科学版）》2005年第2期25-29,共5页Journal of Nanjing Tech University(Natural Science Edition)

基　　金：江苏省自然科学基金资助项目(BK2002019)

摘　　要：碳源、氮源、各种维生素和金属离子对微生物培养有重要影响。先采用单因素,后采用复合因素的方式,确定碳源和氮源,然后采用中心复合设计分析法对地衣芽孢杆菌(Bacilluslicheniformis)NG521-1的培养基主要成分进行分析和优化,根据预测最优发酵水平的配比,调整培养基,使发酵液中菌体生长量大大提高,由原来0 35×108个/mL提高到1 2×108 个/mL。Microorganism growth is greatly effected by carbon sources , nitrogen sources, vitamin and metal ions. With the investigation of single factor, the components of carbon source and nitrogen source were firstly designed respectively , and the optimized cultural medium was decided by the central complex design method then, with which the prediction of the optimized medium has been made for the culture of B. licheniformis NG521-1. Fermentation was conducted with the predicted medium, the grown population of bacteria per unit broth increased sharply, from 3.5×10~7 cfu/mL up to 1.2×10~8 cfu/mL.

关 键 词：地衣芽孢杆菌 大肠杆菌 发酵工艺 培养方法 分析方法 碳源 

分 类 号：TQ92[轻工技术与工程—发酵工程]