射线照射对胰腺癌细胞凋亡及bcl-2、bax基因表达的影响  

Relationship between irradiation dose and expressions of bcl-2,bax genes in apoptosis process of pancreatic carcinoma cells in vitro

作　　者：尹立杰[1] 徐晓颖[2] 丁田贵[1] 朱晓钰[3] 纪军[3] 邹丽娟[2] 

机构地区：[1]大连大学医学院附属大连中心医院放疗科,116033 [2]大连医科大学附属二院肿瘤科 [3]大连大学医学院附属大连中心医院实验室,116033

出　　处：《中华放射医学与防护杂志》2005年第2期127-129,共3页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的研究不同剂量的γ射线对人胰腺癌细胞凋亡及bcl2,bax基因表达的影响。方法利用不同剂量的γ射线对体外培养的胰腺癌Panc1细胞进行照射,采用PI和AnnexinⅤPI法定量检测细胞凋亡,流式细胞仪检测照射后胰腺癌细胞Panc1的Bcl2、Bax基因表达水平。结果胰腺癌panc1细胞凋亡百分率在一定剂量范围内(≤15Gy)随着照射剂量的提高而增大,在一定时间范围内(≤24h),随着时间的延长而增大。照射组细胞凋亡相关基因Bcl2表达较对照组明显降低,两组相比差异有统计学意义(P<005);照射组bax基因的表达较对照组明显升高,两组相比差异有统计学意义(P<005)。结论γ射线诱导胰腺癌panc1细胞凋亡具有剂量依赖性和时间相关性,bcl2和bax在胰腺癌细胞凋亡调节过程中起着重要作用,不同剂量γ射线照射胰腺癌细胞时Bcl2和Bax表达水平也不相同,通过降低bcl2的表达及提高bax表达来诱导胰腺癌细胞发生凋亡有可能是γ射线杀伤肿瘤细胞的机制之一。Objective To explore the relationship between irradition dose and expressions of bcl-2,bax genes in apoptosis process of pancreatic carcinoma cells. Methods Apoptosis was detected with PI and AnnexinⅤ/PI methods and bcl-2,bax proteins in Panc-1 cells after gamma-irradiation were determined with flow cytometry. Results The results showed that the apoptotic rate increased along with the increase of dose and time within a certain range (≤15 Gy,≤24 h). The expression of bcl-2 was significantly lower in the irradiated cells than that in the control cells (P<0.05),but that of bax was higber in the former than that in the latter (P<0.05). Conclusion Gamma-radiotherapy can induce apoptosis in pancreatic carcinoma cells through down-regulation of the expression of bcl-2 and up-regulation of the the expression of bax.;

关 键 词：BAX基因表达 癌细胞凋亡 bcl一2 细胞凋亡相关基因 PANC-1 PANC-1 bcl-2 ANNEXIN 流式细胞仪检测 Bcl-2表达 胰腺癌细胞 不同剂量 基因表达水平 细胞凋亡调节 杀伤肿瘤细胞 时间相关性 剂量依赖性 BAX表达 Γ射线照射 癌细胞发生 

分 类 号：R735.9[医药卫生—肿瘤]