培养的人动脉平滑肌细胞诱导高密度脂蛋白氧化修饰  

Oxidative Modification of High Density Lipoprotein Induced by Cultured Human Arterial Smooth Muscle Cell

作　　者：江渝[1] 刘红[2] 彭家和[1] 叶治家[1] 何凤田[1] 董燕麟[1] 

机构地区：[1]第三军医大学基础部生物化学与分子生物学教研室,重庆市400038 [2]第三军医大学新桥医院血液科,重庆市400037

出　　处：《中国动脉硬化杂志》2005年第1期17-19,共3页Chinese Journal of Arteriosclerosis

基　　金：重庆市医学科学研究基金项目 ( 0 0 2 0 0 5 )

摘　　要：目的　探讨高密度脂蛋白氧化修饰发生的机制。方法　利用体外培养的人动脉平滑肌细胞与人血浆高密度脂蛋白共同温育后 ,用琼脂糖凝胶电泳方法观察高密度脂蛋白电泳迁移率 ,紫外光分光光度法测定高密度脂蛋白共轭二烯的含量 ,利用荧光分光光度法测定其硫代巴比妥酸反应物质的量 ,用可见光分光光度法测定高密度脂蛋白的脂氢过氧化物含量。结果　人血浆高密度脂蛋白与人动脉平滑肌细胞共同培养 4 8h后 ,与天然高密度脂蛋白比较 ,其电泳迁移率明显增加 ,其共轭二烯、硫代巴比妥酸反应物质及脂氢过氧化物的含量均比天然高密度脂蛋白显著增加 (P <0 .0 1)。Aim To observe the mechanism of oxidative modification of high density lipoprotein induced by cultured human arterial smooth muscle cell. Methods The oxidative modification of high density lipoprotein was identified by agarose gel electrophoresis mobility change, absorbance at 234 nm by ultraviolet light, level of lipid hydroperoxide by visible light and fluorescence of thiobarbuturic acid reaction substance of human plasma high density lipoprotein. Results The electrophoretic mobility of high density lipoprotein was increased and absorbance at 234 nm, lipid hydroperoxide and thiobarbuturic acid reaction substance of high density lipoprotein incubated by cultured human arterial smooth muscle cell were significantly higher than that of the native high density lipoprotein. Conclusion Oxidative modification of high density lipoprotein could be induced by cultured human arterial smooth muscle cell.

关 键 词：病理学与病理生理学 培养细胞诱导高密度脂蛋白氧化修饰 蛋白电泳迁移率 高密度脂蛋白 人动脉平滑肌细胞 氧化修饰 细胞培养 

分 类 号：R363[医药卫生—病理学]