放疗诱导鼻咽癌细胞株CNE1热休克蛋白高表达  

Radiation induced HSPs overexpression in nasopharyngeal carcinoma cell line CNE1

作　　者：冯雪萍[1] 陈主初[1] 肖志强[1] 易红[1] 李建玲[1] 杨轶轩[1] 王郁杰[1] 诸葛勤[1] 朱果[1] 张鹏飞[1] 肖健云[2] 王行炜[2] 

机构地区：[1]中南大学湘雅医院卫生部肿瘤蛋白质组学重点实验室,湖南长沙410008 [2]中南大学湘雅医院耳鼻咽喉科,湖南长沙410008

出　　处：《中国耳鼻咽喉颅底外科杂志》2005年第2期81-84,共4页Chinese Journal of Otorhinolaryngology-skull Base Surgery

基　　金：国家重点基础研究发展规划项目(973)(2001CB510207);国家自然科学基金资助项目(30000028);教育部跨世纪优秀人才培养计划基金(教计函[2002]48);湖南省科技厅一般项目(03SSY4019);湖南省自然科学基金资助项目(03JJY4021);湖南省卫生厅重点科研项目(Z02-04)。

摘　　要：目的鼻咽癌是一种以放射治疗为主的头颈肿瘤,但部分鼻咽癌对放疗耐受。为了解鼻咽癌放疗耐受机制,本实验拟初步探讨人高分化鼻咽癌细胞株CNE1放疗后的蛋白质改变。方法利用固相pH梯度二维凝胶电泳,建立CNE1放疗前后总蛋白质2DE图谱,利用MALDI TOF MS及数据库搜索初步分析和鉴定部分放疗前后差异蛋白质点。结果高分子量酸性蛋白质在CNE1放疗后20min高表达,其中大多为热休克蛋白质HSPs(heatshockproteins,HSPs)。结论放射能诱导CNE1的热休克蛋白高表达,它们可能与鼻咽癌放疗耐受有关。Objective Nasopharyngeal carcinoma (NPC) is a common head and neck cancer treated mainly by radiotherapy. But some of the NPC are radiation resistance. To understand the intrinsic mechanisms of NPC radiation resistance, the alterations were analyzed for the protein expression of human well-differentiated NPC cell line CNE1 after radiation, which is relatively resistant to radiotherapy. Methods The total protein 2-DE maps of CNE1 cells before and after radiation were established by Two-dimensional gel electrophoresis (2-DE), and some of the differential expressional proteins were identified by MALDI-TOF-MS and database searching. Results The high molecular weight acid proteins were over-expressed 20 min after radiation, most of which are heat shock proteins (HSPs). Conclusion Radiation can induce over-expression of HSPs in CNE1, and these over-expressed HSPs may be relative to CNE1 irradiation resistance.

关 键 词：鼻咽肿瘤/放射疗法 热休克蛋白类 细胞株CNE1 2-DE MALDI—TOF—MS 蛋白质组 

分 类 号：R739.63[医药卫生—肿瘤] Q51[医药卫生—临床医学]