应用快速引物原位标记技术检测鼻咽癌染色体异常(英文)  

Detection of the chromosomes numerical aberration in frozen sections of nasopharyngeal carcinoma by rapid primed in situ labeling

作　　者：谢民强[1] 黄郁林[1] 肖健云[2] 赵素萍[2] 陈朝晖[3] 王承龙[2] 

机构地区：[1]中山大学附属第三医院耳鼻咽喉科,广东广州510630 [2]中南大学湘雅医院耳鼻咽喉科,湖南长沙410008 [3]中南大学湘雅医院中心实验室,湖南长沙410008

出　　处：《中国耳鼻咽喉颅底外科杂志》2005年第2期85-91,共7页Chinese Journal of Otorhinolaryngology-skull Base Surgery

基　　金：广东省科技计划项目(No.730711);广东省医学科学研究基金(No.1999190);广东省自然科学基金(No.36645)联合资助。

摘　　要：目的探讨快速引物原位标记技术检测鼻咽癌染色体异常的可行性。方法采用快速引物原位标记技术,分别以人3号和7号染色体特异性寡核苷酸作引物,检测15例鼻咽癌和5例鼻咽正常冰冻组织切片细胞染色体,染色体异常标准以标记信号≤1的细胞比例≥65%时视为染色体丢失,标记信号≥3的细胞比例≥6.5% 作为染色体拷贝数增加。结果鼻咽癌组织细胞3号染色体标记率为88.6%,10例(66.7%)染色体拷贝数增加;7号染色体标记率87.4%,5例(33.3%)染色体丢失;其中4例同时存在3号染色体拷贝数增加和7号染色体丢失。正常组织3号和7号染色体标记率分别为92%和91.8%,二倍体细胞分别为43.2%和43.6%,与鼻咽癌比较差异均有统计学意义(P<0.05),未发现三体细胞。结论快速引物原位标记技术可用于鼻咽癌冰冻组织切片中染色体的检测,染色体数目的改变可能有助于鼻咽癌的诊断。Objective The chromosomal aberration is a common event in nasopharyngeal carcinoma (NPC). The currently-used methods to detect chromosomal abnormalities are complicated and of limited clinical value. The purpose of the present study is to explore the feasibility of detecting chromosomal abnormalities in NPC by rapid primed in situ labeling (PRINS). Methods The copy changes of Chromosome 3 and 7 in the frozen section of 15 cases of NPC and five cases of normal nasopharyngeal mucosa were detected using rapid PRINS and oligonucleotide primers specific for chromosome 3 and 7. The standards of the chromosomes numerical aberration are as the following: the ratio of cells with one or no labeling signals at 65% or more was defined as the losses of chromosome; the ratio of cells with three or more labeling signals at 6.5% or more was defined as the gain of chromosome copy numbers. Results Of 15 cases of carcinoma tissues, the labeling rate of chromosome 3 and 7 was (87.4%) and (88.6%) respectively. The gain of chromosome 3 copy numbers was observed in ten cases ((66.7%)) and the loss of chromosome 7 was detected in five cases ((33.3%)). Four cases of them had both the gain of chromosome 3 copy numbers and the loss of chromosome 7. In normal nasopharyngeal tissues the labeling rate of chromosome 3 and 7 was 92% and (91.8%) respectively, and diploid cells were (43.2%) and (43.6%) respectively, without trisomy. The difference of diploid cells between primary NPC and normal nasopharyngeal mucosa was statistically significant (P<(0.05)). Conclusion The results indicate that rapid PRINS method can be used to detect interphase chromosomes in frozen tissue sections and chromosomes numerical aberration, which may be helpful to the diagnosis of NPC.

关 键 词：鼻咽肿瘤/遗传学 染色体异常 快速引物原位标记 冰冻切片 

分 类 号：R739.63[医药卫生—肿瘤] R596.1[医药卫生—临床医学]