采用TOPO-TA克隆法建立多肽:N-乙酰氨基半乳糖转移酶基因家族cDNA文库  

Foundation of Polypeptide: N-acetylgalactosaminyltransferases cDNA Library by TOPO-TA Cloning

作　　者：史宁[1] 贾伟[1] 吴士良[1] 

机构地区：[1]苏州大学医学院生化和分子生物学教研室

出　　处：《江苏大学学报（医学版）》2005年第2期104-106,共3页Journal of Jiangsu University：Medicine Edition

基　　金：苏州大学医学发展基金项目(2003007)

摘　　要：目的:通过实验方法建立多肽N-乙酰氨基半乳糖转移酶基因家族cDNA文库,欲以此为基础对人体不同肿瘤细胞中的该基因家族进行表达谱分析。方法:主要为PCR和TOPO-TA克隆技术。结果:通过电泳图谱初步证实了这一家族的不同成员均已得到有效的扩增,并获得了相应的片段库。结论:多肽:N-乙酰氨基半乳糖转移酶基因家族cDNA被成功扩增,并建立了该基因家族的cDNA文库。Objective: To make the cDNA library by TOPO-TA cloning in order to analyze the differences of N-acetylgalactosaminyltransferases gene expression profile in different human tumor cells. Methods: Mainly by means of PCR and TOPO-TA cloning. Results: Through patterns of electrophoresis, it was rudimentarily proved that N-acetylgalactosaminyltransferases gene family cDNA had been successfully amplified and the cDNA library had also been made. Conclusion: N-acetylgalactosaminyltransferases gene family cDNA has been amplified successfully and the cDNA library has been made by means of TOPO-TA cloning.

关 键 词：多肽 N-乙酰氨基半乳糖转移酶基因家族 PCR TOPO-TA克隆 CDNA文库 

分 类 号：R-33[医药卫生]