二烯丙基二硫对人结肠癌HT-29细胞周期的阻滞作用  

Arrest Effect of Diallyl Disulfide on Cell Cycle in Human Colon Cancer Cell

作　　者：黄幼生[1] 苏琦[1] 周秀田[1] 苏坚[1] 廖前进[1] 解娜[1] 葛玲[1] 

机构地区：[1]南华大学肿瘤研究所,湖南衡阳421001

出　　处：《肿瘤学杂志》2005年第2期103-106,共4页Journal of Chinese Oncology

摘　　要：[目的]探讨二烯丙基二硫(DADS)对人结肠癌HT鄄29细胞周期的阻滞作用及其分子机制。[方法]应用MTT法及细胞计数法测量HT鄄29细胞生长抑制,流式细胞术检测细胞时相分布,免疫细胞法测定p21、C鄄myc、CyclinE表达。[结果]MTT法显示,不同浓度DADS作用HT鄄29细胞12、24、48h后,细胞生长抑制率及细胞群体倍增时间呈浓度、时间依赖性增加。流式细胞仪分析,30、60μmol/LDADS阻滞HT鄄29细胞在G1期,与对照组相比,可使G1期细胞增加约2倍,而90、120μmol/LDADS显著地将细胞阻滞在G2/M期。免疫细胞化学分析表明在细胞周期阻滞的同时有p21waf1蛋白表达上调,CyclinE、C鄄myc蛋白表达下降。[结论]DADS对HT鄄29细胞的抑制增殖作用可能与细胞周期阻滞有关,DADS对HT鄄29细胞周期阻滞的分子机制可能与调节p21waf1、CyclinE、C鄄myc表达相关。To investigate the arrest effect of diallyl disulfide(DADS) in the cell cycle of human colon cancer HT-29 cells and its molecular mechanisms. HT-29 cells growth inhibition were measured by MTT assay and cell counting. Phase distribution of cell cycle was analyzed by flow cytometry. Expression of p21waf1,C-myc,Cyclin E was determined by immunohisto-chemistry analysis. MTT assay showed that DADS significantly inhibited HT-29 cells and exhibited a time, dose-dependent model. after adding various concentration of DADS for 12, 24, 48h. Flow cytometry analysis revealed that treating HT-29 cells with 60μmol/L DADS accumulated cells in the G1 phase,however, 90, 120μmol/L DADS caused G2/M arrest. The proportion of cells in the G1 phase after treatment with 30, 60μmol/L DADS for 24h was almost 2-folds that occurring in untreated cells. Immunohistochemistry analysis revealed that DADS increased the amount associated with p21waf1 and decreased the levels of C-myc, Cyclin E protein. [Conclusion] The antiproliferation property of diallyl disulfide (DADS) in cultured human colon carcinoma HT-29 cell line relates to its ability to arrest cell cycle arrest of HT-29 cells by DADS may be related to the expression of p21waf1, C-myc, Cyclin E protein.

关 键 词：二烯丙基二硫 结肠肿瘤 HT-29细胞 细胞周期 CYCLIN E蛋白 

分 类 号：R73-361[医药卫生—肿瘤]