胞外弹性蛋白酶的理化特性及其影响因素  

Studies on Properties of Extracellular Elastase from Pseudomonas.sp

作　　者：贺稚非[1] 陈宗道[1] 李洪军[1] 刘鹭[1] 高兆建[1] 宫春波[2] 

机构地区：[1]西南农业大学食品科学学院,重庆400716 [2]莱阳农学院,莱阳500126

出　　处：《食品与发酵工业》2005年第4期10-13,共4页Food and Fermentation Industries

基　　金：国家 8 63计划 (No .2 0 0 2AA2 4 80 31 )资助项目

摘　　要：分离得到的假单胞菌(Pseudomonas sp) 菌株具有较强的分泌胞外弹性蛋白酶的能力。经微生物发酵方法生产的弹性蛋白酶,经过盐析、透析、DEAE SephadexA2 5离子交换层析、SephadexG75凝胶过滤层析等纯化步骤,从其发酵液中得到了均一的酶制品。研究结果表明,酶的最适作用温度为5 0℃;在硼砂-硼酸缓冲液中最适作用pH为8 0左右;酶在碱性环境下( pH7 0～12 0 )稳定性较好;在37℃以下,酶的稳定性较高。超过6 0℃。An extracellular Elastase secreted by a Pseudomonas sp strain was isolated in the laboratory A crude enzyme solution from the culture fluid of Pseudomonas sp was purified to SDS-PAGE homogeneity by (NH_4)_2SO_4 precipitation, Anion-exchange chromatography of DEAE-Sephadex A25 and Gel-filtration chromatography on Sephadex G75 The elastase showed two peaks by HPLC and its molecular weight was estimated to be 32 ku by SDS-PAGE The optimum condition for elastolytic activity was pH7 8, temperature 50℃ The enzyme was stable in the alkaline condition (pH7 0～12) This enzyme is stable below 37℃ while it lost nearly all its activity within short time above 60℃

关 键 词：弹性蛋白酶 理化特性 SEPHADEX 离子交换层析 凝胶过滤层析 假单胞菌 发酵方法 DEAE 研究结果 作用温度 碱性环境 稳定性 微生物 酶制品 发酵液 缓冲液 透析 

分 类 号：TQ925[轻工技术与工程—发酵工程]