硒酸钠对人前列腺癌细胞PC-3细胞株活力和增殖的影响  

Effect of sodium selenate on the viability and proliferation of PC-3 cell line, a kind of human prostate cancer cell

作　　者：张保国[1] 杨劲松[1] 姚乐申[2] 沈苏南[3] 

机构地区：[1]南京医科大学附属南京市第一医院肿瘤科,江苏南京210006 [2]南京大学医学院附属鼓楼医院泌尿外科,江苏南京210008 [3]南京大学医学院细胞生物学教研室,江苏南京210093

出　　处：《中国癌症杂志》2005年第2期153-155,共3页China Oncology

摘　　要：目的:研究硒酸钠(一种主要的无机含硒化合物)对人前列腺癌细胞系PC3生长和增殖的影响。方法:用硒酸钠对体外培养的人前列腺癌细胞PC3进行药物处理,然后用MTT法测定细胞的活力,用3HTdR掺入法测定细胞的增殖情况。结果:24小时作用时,硒酸钠中剂量组A值显著低于对照组,高剂量组A值与对照组有极显著差异。48小时作用时,硒酸钠低剂量组A值显著低于对照组,中剂量和高剂量组A值与对照组有极显著差异;48小时的硒酸钠处理抑制PC3细胞的增殖,中剂量组与对照组相比有显著差异,高剂量组与对照组有极显著差异。结论:硒酸钠可抑制PC3细胞的活力,抑制细胞增殖,并且这两种作用都呈剂量依赖效应。Purpose:To study the effect of sodium selenate on the viability and proliferation of PC-3 cell, a kind of human prostate cancer cell. Methods:Sodium selenate was administered to PC-3 cells, and MTT assay and ^(3)H-TdR adulterated assay were used to estimate the viability and proliferation of cell. Results:① When cells were treated with Sodium selenate for 24 h, the optical density (A) of middle-dose group decreased significantly, and the A of the high-dose group decreased dramatically. When cells were treated for 24 h, the A of the low-dose group was significantly lower than that of the control group, while the A of the middle-dose and high-dose groups was much lower than control.② When cells were treated for 24 h, the proliferation of middle-dose group decreased, and that of high-dose group decreased markedly. Conclusions:Sodium selenate can inhibit the viability and proliferation of PC-3 cells, and these actions occur in a dose-dependant manner.

关 键 词：硒酸钠 PC-3细胞系 MTT法 ^3H-TdR掺入法 

分 类 号：R737.25[医药卫生—肿瘤]