血管内皮生长因子反义寡核苷酸抑制甲状腺癌血管生成的效应  

The inhibitory effect of vascular endothelial growth factor antisense oligonucleotides in angiogenesis of thyroid carcinoma

作　　者：张海燕[1] 高大新[2] 李萍[3] 任路平[1] 曹翠萍[1] 刘国良[1] 

机构地区：[1]中国医科大学附属第一医院内分泌科,沈阳110001 [2]中国医科大学附属第一医院骨科,沈阳110001 [3]沈阳军区总医院内分泌科

出　　处：《中华内科杂志》2005年第4期280-284,共5页Chinese Journal of Internal Medicine

摘　　要：目的探讨反义寡核苷酸(ASODN)抑制甲状腺癌细胞血管内皮生长因子(VEGF)表达及内皮细胞生长的效应。方法设计合成靶向VEGF的ASODN转染人髓状甲状腺癌细胞系(TT)细胞,并制备相应条件培养基作用内皮细胞ECV304,设正义寡核苷酸(SODN)和空白对照组进行比较。观察细胞生长状态,RT PCR、免疫细胞化学法检测TT细胞VEGFmRNA和蛋白表达,四氮唑蓝法检测TT和ECV304细胞生长抑制率(IR),流式细胞仪、吖啶橙/溴化乙锭染色法检测ECV304细胞凋亡状态。结果ASODN组TT细胞VEGFmRNA和蛋白表达显著低于SODN和对照组(P<0.01),但IR差异无统计学意义(P>0.05);各转染组ECV304细胞IR差异亦无统计学意义(P>0.05);而经各ASODN组TT细胞条件培养基作用的ECV304细胞生长明显受抑,IR(分别为0.21±0.03、0.31±0.01、0.42±0.22)显著高于SODN组(0.05±0.03,P<0.01),并伴明显细胞凋亡,上述效应呈浓度依赖性。结论ASODN可通过特异性封闭甲状腺癌细胞VEGF表达,抑制内皮细胞生长,干扰肿瘤血管生成。Objective To investigate the inhibitive effect of antisense oligonucleotide (ASODN) on vascular endothelial growth factor (VEGF) expression and endothelial cell growth in thyroid carcinoma.Methods Targeted ASODN of VEGF was designed and synthesized, then transfected to TT (medullary thyroid carcinoma) cell line and the culture supernatant was collected in which ECV304 (endothelial cell line) was seeded. At the same time positive control [sense oligonucleotides (SODN) group] and normal control were set for comparison. Cell growth condition was observed under microscope. RT-PCR and immuocytochemistry were used for detection of VEGF mRNA and protein expression in TT cells. MTT assay was used for cell growth inhibition ratio (IR) of TT and ECV304 cells, flow cytometry (FCM) for apoptotic index (AI) of ECV304 cells and acridine orange/ethidium bromide (AO/EB) staining for apoptotic morphology of ECV304 cells.Results As compared with positive and normal control groups, VEGF mRNA and protein expressions in TT cells of ASODN transfection groups were obviously decreased (P<0.01). Cell growth was not influenced apparently in ECV304 cells with direct ASODN administration, but ECV304 cell growth in ASODN conditioned medium was significantly inhibited and IR (0.21±0.03、0.31±0.01、0.42±0.22) was significantly higher than that of SODN group (0.05±0.03, P<0.01), with the presence of apparent apoptosis. The effect mentioned above was in a dose-dependent manner.Conclusion ASODN can suppress endothelial cell growth and inhibit tumor angiogenesis possibly by specifically blocking VEGF expression in thyroid carcinoma.

关 键 词：血管内皮生长因子(VEGF) 效应 甲状腺癌 酸抑制 ASODN ECV304细胞 内皮细胞生长 免疫细胞化学法 细胞生长抑制率 条件培养基 反义寡核苷酸 正义寡核苷酸 细胞生长状态 VEGF表达 肿瘤血管生成 腺癌细胞 蛋白表达 mRNA 细胞凋亡 

分 类 号：R736.1[医药卫生—肿瘤]