核转录因子κBp65蛋白及其抑制蛋白IκBα表达与食管鳞癌浸润转移的关系  

The Relationship between the Expressions of p65 Proteins of Nuclear Transcription Factor kappa B (NF-κB) and Its Suppressor Protein-IκBα and the Infiltration and Metastasis of Esophageal Squamous Cell Carcinoma

作　　者：李道明[1] 李珊珊[1] 赵志华[1] 任秀花[2] 高冬玲[1] 曹静[1] 李海梅[1] 闫爱华[2] 

机构地区：[1]郑州大学第一附属医院病理科 [2]郑州大学基础医学院癌前期研究室

出　　处：《中国肿瘤临床》2005年第9期497-500,512,共5页Chinese Journal of Clinical Oncology

基　　金：河南省自然科学基金项目资助(编号:0411043200)

摘　　要：目的:探讨核转录因子κB(NF-κB)p65蛋白及其抑制蛋白IκBα表达与食管鳞癌浸润转移的关系。方法:用免疫组化SP法检测了61例食管鳞癌中NF-κBp65、IκBα蛋白的表达。结果:p65、IκBα蛋白的胞浆表达与食管鳞癌的组织学分级、浸润深度和淋巴结转移无关,但p65蛋白的胞核表达与食管鳞癌的组织学分级、浸润深度和淋巴结转移均呈正相关(P值均<0.01),IκBα蛋白的胞核表达与食管鳞癌的淋巴结转移有关(P<0.05)。在食管鳞癌中IκBα蛋白的胞浆和胞核阳性率均高于p65蛋白,但差异均无统计学意义,也无相关关系。结论:NF-κBp65蛋白的胞核表达与食管鳞癌的分级、浸润和淋巴结转移有关,IκBα蛋白的胞核表达与食管鳞癌的淋巴结转移有关。Objective: To study the relationship between the expressions of p65 proteins of nuclear transcription factor kappa B(NF-κB)and its suppressor protein-IκBαand the infiltration and metastasis of esophageal squamous cell carcinoma. Methods: S-P immunohistochemical method was used to detect the expressions of p65 proteins of NF-κB and IκBα protein in the specimens of 61 cases with esophageal squamous cell carcinoma. Results: There was no correlationship between the positive expression of p65, IκBα protein in plasma and the tumour grade, depth of infiltration and lymphatic metastasis of esophageal squamous cell carcinoma, but there was a positive correlationship between the positive rate of p65 expression in nuclei and the tumour grade, depth of infiltration and lymphatic metastasis of esophageal squamous cell carcinoma(P<0.01) and between the positive rate of IκBα protein expression in nuclei and the lymphatic metastasis of esophageal squamous cell carcinoma(P<0.05). In the esophageal squamous cell carcinoma, the positive rates of IκBα protein in plasma and nuclei were both higher than that of p65 protein, but there was no significant difference and correlationship between them. Conclusion: The expression of p65 proteins of NF-κB in nuclei may be correlated with tumour grade, infiltration and lymphatic metastasis of esophageal squamous cell carcinoma. The expression of IκBαproteins in nuclei may be correlated with the lymphatic metastasis of esophageal squamous cell carcinoma.

关 键 词：癌浸润转移 核转录因子 抑制蛋白 白及 IκBα蛋白 淋巴结转移 NF-κBp65 P65蛋白 食管鳞癌 免疫组化SP法 组织学分级 浸润深度 核表达 相关关系 正相关 阳性率 统计学 胞浆 

分 类 号：R735.1[医药卫生—肿瘤]