牛磺酸对大鼠肝纤维化的防治作用及机制的初步观察  

Observation of the Promotion Effect Taurine on Hepatic Stellate Cell's Apoptosis in Rat Hepatic Fibrosis Model

作　　者：梁健[1] 张锡流[2] 杨光业[3] 庞玉生[4] 袁海锋[1] 梁劲松[2] 黄仁彬[4] 

机构地区：[1]广西中医学院附属瑞康医院消化内科,南宁530011 [2]广西中医学院第一附属医院 [3]广西医学情报所 [4]广西医科大学

出　　处：《四川大学学报（医学版）》2005年第3期365-367,共3页Journal of Sichuan University(Medical Sciences)

基　　金：广西壮族自治区自然科学基金 (No.0 14 40 16)资助

摘　　要：目的　观察牛磺酸对四氯化碳(CCl4 )诱导的大鼠肝纤维化模型中星状细胞凋亡的影响及其保护机制。方法　将实验动物分为正常对照组、模型组和牛磺酸组,采用CCl4 诱导大鼠肝纤维化模型,研究牛磺酸对血清丙氨酸氨基转移酶(AL T)、透明质酸(HA)、型前胶原(PC )、肝药酶和抗转化生长因子β1 (TGF- β1 )以及对激活的肝星状细胞凋亡的影响。结果　与模型组比较,牛磺酸组AL T、HA、PC 水平降低,肝细胞色素P4 5 0、细胞色素b5含量提高,TGF- β1 的表达明显减少,同时促进了激活的肝星状细胞凋亡,明显减轻纤维化程度。结论　牛磺酸有抑制TGF-β1 的表达,促进激活的肝星状细胞凋亡的作用,具有明显的抗肝纤维化作用。Objective To observe the effect of taurine on hepatic stellate cell's apoptosis induced by carbon tetrachloride (CCl_4) in rats and to study its protective mechanisms. Methods CCl_4-induced rat hepatic fibrosis was treated by taurine. Serum alanine aminotransferase (ALT), plasma protein, hyaluronic acid (HA), procollagen Ⅲ (PCⅢ), hepatic microsomal drug-metabolizing enzyme and anti-transforming growth factor β_1 (TGF-β_1) were determined. In addition, hepatic stellate cell's apoptosis and the pathological changes of liver tissue were observed under light microscope. Results The activity of serum ALT and the levels of serum HA, PCⅢ were markedly reduced by taurine treatment. The hepatic cytochrome P450 (Cyt.P450) and cytochrome b_5 (Cyt b_5) contents were increased by the same treatment. In addition, taurine could significantly inhibit the expression of TGF-β_1, promote the hepatic stellate cell's apoptosis, and relieve hepatic fibrosis. Conclusion Taurine fulfills a role in promoting hepatic stellate cell's apoptosis in the case of hepatic fibrosis, it mitigates the liver injury, decreases the expression of TGF-β_1, and relieves hepatic fibrosis.

关 键 词：牛磺酸 肝纤维化 肝星状细胞凋亡 

分 类 号：R575.2[医药卫生—消化系统]