EGFR、PCNA、N-myc和P16基因蛋白产物在髓母细胞瘤中的表达  

Expression of EGFR,PCNA,N-myc and P16gene proteins in human medulloblastoma

作　　者：买买提力[1] 张巍[2] 郭怀荣[1] 鲍遇海[1] 柳琛[1] 石鑫[1] 薛少杰[1] 阿布里米提 陈昭伦[2] 

机构地区：[1]新疆医科大学第一附属医院神经外科,830054 [2]新疆医科大学第一附属医院病理科,830054 [3]新疆哈密地区医院神经外科

出　　处：《中华神经外科杂志》2005年第5期286-290,共5页Chinese Journal of Neurosurgery

摘　　要：目的探讨EGFR、Nmyc、PCNA和P16基因在髓母细胞瘤发生中的作用,以及上述基因蛋白检测对髓母细胞瘤的诊断、病理分型和年龄的临床意义。方法用免疫组织化学LSAB法检测四种基因蛋白的产物在41例人脑髓母细胞瘤中的表达,分析与髓母细胞瘤病理分型和年龄间的关系。结果EGFR、Nmyc和PCNA蛋白在髓母细胞瘤中高度表达,P16蛋白有明显的缺失,分别为56.10%、60.98%、65.89%、58.54%。EGRF蛋白在两种主要病理类型中的表达差异有统计学意义(P<0.01),P16蛋白在两组不同年龄组间的差异有统计学意义(P<0.05)。结论上述四种基因在髓母细胞瘤的发生中可能起重要作用,EGFR和P16蛋白的检测对病理分型和预后判断有临床实际应用意义。Objective To investigate the significance of EGFR, N-myc, PCNA and P16 genes in the development, diagnosis and pathological classification of human medulloblastoma, we examined their expressions in tumor tissue. Methods The expressions of EGFR, N-myc, PCNA and P16 gene proteins were detected immunohistochemically in 41 patients with medulloblastoma. The relationship between pathological classifications and ages were determined. Results Expression of EGFR,N-myc and PCNA, deletion of P16 were 56.10%,60.98%,65.8% and 58.54% in human medulloblastoma respectively. It showed significant difference(P<0.01) between the two main pathological classifications in EGFR and between the two different age groups in p16(P<0.05). Conclusions The genes mentioned above may play an important role in the development of medulloblastoma, especially EGFR and P16. The detection of these proteins has a positive significance on malignancy determination of medulloblastoma.

关 键 词：P16基因 EGFR myc 蛋白产物 免疫组织化学LSAB法 P16蛋白 病理分型 脑髓母细胞瘤 PCNA蛋白 不同年龄组 蛋白检测 临床意义 基因蛋白 表达差异 病理类型 应用意义 预后判断 统计学 F蛋白 

分 类 号：R739.4[医药卫生—肿瘤]