一种新型抗菌肽Cecropin B真核表达载体的构建  

Construction of the Eukaryotic Recombinant Expression Plasmid for a Novel Cecropin B

作　　者：胡仲秋[1] 樊钰虎[1] 冯瑄[1] 杨晓燕[1] 边六交[1] 

机构地区：[1]西北大学基因工程药物研究开发中心,陕西西安710069

出　　处：《生物技术通讯》2005年第3期268-270,共3页Letters in Biotechnology

基　　金：陕西省科技攻关项目(2003K10-G58)

摘　　要：通过重叠区扩增法人工设计和合成一种新型抗菌肽CecropinB基因,按正确的阅读框架定向克隆至巴斯德毕赤酵母的高效表达载体pPIC9K上。经PCR鉴定及序列分析,所转化的大肠杆菌DH5α菌落中含有插入CecropinB基因的重组质粒pPIC9K-CB,表明成功构建了新型抗菌肽CecropinB表达载体pPIC9K-CB。A novel antibacterial peptide cecropin B gene was designed and artificially synthesized through using gene splicing by overlap extension (gene SOEing) method. After this cecropin B gene was subcloned into plasmid pPIC9K, a novel recombinant expression plasmid pPIC9K-CB, which can be efficiently expressed in Pichia pastoris, can be obtained. By using PCR and sequence analyses, it can be proved that all the tested clones contain this novel cecropin B gene, and the novel cecropin B expression plasmid pPIC9K-CB is successfully constructed.

关 键 词：CECROPIN 抗菌肽 真核表达载体 构建 巴斯德毕赤酵母 高效表达载体 PCR鉴定 人工设计 定向克隆 序列分析 大肠杆菌 重组质粒 B基因 重叠区 CB 菌落 

分 类 号：Q516[生物学—生物化学] S511.03[农业科学—作物学]