刺五加多糖抗肿瘤作用与机理的实验研究  

Antitumor action and mechamism of acanthopanax santicosus polysaccharides

作　　者：佟丽[1] 黄添友[1] 梁谋[1,2] 吴波[1,2] 梁念慈[1,2] 李吉来[1] 

机构地区：[1]第一军医大学中西医结合研究所 [2]广东医学院

出　　处：《中国药理学通报》1994年第2期105-109,共5页Chinese Pharmacological Bulletin

基　　金：全军青年科学基金

摘　　要：观察刺五加多糖（ＡＳＰＳ）对小鼠肉瘤Ｓ１８０细胞、人白血病Ｋ５６２细胞体外增殖的影响，发现ＡＳＰＳ对两种细胞的增殖均有强烈抑制作用，半数有效抑制浓度分别为０．３８ｇ·Ｌ－１和０．２８ｇ·Ｌ－１。为探讨其抗肿瘤机理，对Ｓ１８０细胞膜成分进行了分析，结果显示ＡＳＰＳ与细胞接触２４ｈ，引起细胞膜唾液酸含量升高，膜磷脂含量降低，膜磷脂脂肪酸组成发生明显改变（Ｃ２０：０、和Ｃ１４：０降低），与对照组相比，这些变化均有显著差异（Ｐ＜０．０５或Ｐ＜０．０１）；但膜胆固醇含量、膜流动性（Ｃ／Ｐ比值、Ｕ／Ｓ比值）及膜中性脂脂肪酸组成不受影响。将ＡＳＰＳ与Ｓ１８０细胞膜在适当条件下一同温育，发现ＡＳＰＳ干扰膜的肌醇磷脂代谢，明显抑制磷脂酰肌醇转换。上述结果提示ＡＳＰＳ的抗癌机理与膜生化特性改变有关，其中对膜磷脂含量。The effect of ASPS on cell proliferation in mice sarcoma S180 cells and human chronic myelogenous leukemia K562 cells was observed. We found that ASPS strongly inhibited the proliferation of both cells, the IC50 was 0. 38 g . L-1 and 0. 28 g . L-1 respectively.The analysis of S 180 cell merbranes composition showed that after ASPS acting on cells for 24 hours, the content of sialic acid increased , whilephospholipids decreased and the fatty acid composition of membrane phospholipids changed obviousely , arachidonic acid (C20 :4) and myristicacid (C14: 0)reduced. The changes had significant difference as compared with the controlgroup , but the content of membrane cholesterol ,membrane mobility (Ch

关 键 词：刺五加 多糖 肿瘤 细胞增殖 磷脂 肌醇磷脂代谢 脂肪酸组成 唾藏酸 胆圈醇 

分 类 号：R73－36[医药卫生—肿瘤]