从微量血中快速制备线粒体DNA片段  

Quick Preparation of Mitochondrial DNA Fragments from Micro-blood

作　　者：马晓娟[1] 罗新萍[1] 焦龙华[1] 

机构地区：[1]河南科技大学医学院,河南洛阳471003

出　　处：《河南科技大学学报（医学版）》2005年第2期92-94,共3页Journal of Henan University of Science & Technology:Medical Science

摘　　要：目的建立从微量血中快速制备线粒体DNA(mtDNA)片段。方法取50μl抗凝血和常规酶解法提取的血DNA,同时扩增核DNA(nDNA)上的基因片段和mtDNA上的基因片段,华美公司生产的RedyPCR(tm)微量全血DNA纯化系统进行比较,PCR相对定量分析比较这3种方法提取的mtDNA片段的纯度。结果RedyPCR(tm)微量全血DNA纯化系统得到的mtDNA和常规酶解法提取的mtDNA有nDNA污染,而作者建立的方法获得的mtDNA纯度较高。结论同时与该法简便快捷地排除了nDNA的污染,适合于对mtDNA进行PCR分析和研究。Objective To establish quick preparation of mitochondrial DNA (mtDNA) fragments from micro-blood. Methods 50 μl anticoagulant blood and blood DNA were drawn by routine enzymolysis method to dilate nuclear DNA(nDNA) genetic fragments and the genetic fragments of mitochondrial DNA. The method of Ready PCR(tm) micro-blood DNA purified system were used to compare the purity of mtDNA fragments by PCR relative quantitive analysis. Result The mtDNA obtained by Ready PCR(tm) micro-blood DNA purified system and the mtDNA drawn by routine enzymolysis method had nDNA pollution, and the purity of mtDNA obtained by the author's method was higher. Conclusion The method was used to quickly eliminate nDNA contamination, and is suitable for analysis and research of mtDNA by PCR.

关 键 词：DNA片段 快速制备 线粒体 微量血 mtDNA DNA纯化 基因片段 微量全血 NDNA PCR分析 分析比较 酶解法 提取 抗凝血 系统 纯度 污染 

分 类 号：R394[医药卫生—医学遗传学] R392-33[医药卫生—基础医学]